ArticleProceedings of the National Academy of Sciences of the United States of America2000
The central cytoplasmic loop of the major facilitator superfamily of transport proteins governs efficient membrane insertion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Substrate-binding guides individual melibiose permeases MelB to structurally soften and to destabilize cytoplasmic middle-loop C3.Structure (London, England : 1993) · 2023Article
- Key computational findings reveal proton transfer as driving the functional cycle in the phosphate transporter PiPT.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Isolation and characterisation of transport-defective substrate-binding mutants of the tetracycline antiporter TetA(B).Biochimica et biophysica acta · 2015Article
- A structured loop modulates coupling between the substrate-binding and dimerization domains in the multidrug resistance transporter EmrE.The Journal of biological chemistry · 2015Article
- Efflux pump proteins in antifungal resistance.Frontiers in pharmacology · 2014Review
- Lipid-dependent generation of dual topology for a membrane protein.The Journal of biological chemistry · 2012Article
- Article
- Structural basis of GLUT1 inhibition by cytoplasmic ATP.The Journal of general physiology · 2007Article
- SecY alterations that impair membrane protein folding and generate a membrane stress.The Journal of cell biology · 2007Article
- Study of the five Rickettsia prowazekii proteins annotated as ATP/ADP translocases (Tlc): Only Tlc1 transports ATP/ADP, while Tlc4 and Tlc5 transport other ribonucleotides.Journal of bacteriology · 2006Article
- Structural and functional characterization of AtPTR3, a stress-induced peptide transporter of Arabidopsis.Journal of molecular modeling · 2005Article
- Structural conservation in the major facilitator superfamily as revealed by comparative modeling.Protein science : a publication of the Protein Society · 2004Article
- Role of YidC in folding of polytopic membrane proteins.The Journal of cell biology · 2004Article
- Cytoplasmic domains of the reduced folate carrier are essential for trafficking, but not function.The Biochemical journal · 2002Article
- A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition.The EMBO journal · 2002Article
- Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genes and proteins for solute transport and sensing.The arabidopsis book · 2002Article
- Twelve-transmembrane-segment (TMS) version (DeltaTMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity.Journal of bacteriology · 2001Article
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Abstract
Deletion of 5 residues (Delta5) from the central cytoplasmic loop of the lactose permease of Escherichia coli has no significant effect on expression or activity, whereas Delta12 leads to increased rates of permease turnover after membrane insertion and decreased transport activity, and Delta20 abolishes insertion and activity. By expressing Delta12 or Delta20 in two halves, both expression and activity are restored to levels approximating wild type. Replacing deleted residues with random hydrophilic amino acids also leads to full recovery. However, introduction of hydrophobic residues decreases expression and activity in a context-dependent manner. Thus, a minimum length of the central cytoplasmic loop is vital for proper insertion, stability, and efficient transport activity, because of constraints at the cytoplasmic ends of helices VI and VII. Furthermore, the results are consistent with the idea that the middle cytoplasmic loop provides a temporal delay between insertion of the first six helices into the membrane before insertion of the second six helices.
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