ArticleGenetics2000
Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Selective function of the PDZ domain of Dishevelled in noncanonical Wnt signalling.Journal of cell science · 2022Article
- Article
- The Fog signaling pathway: insights into signaling in morphogenesis.Developmental biology · 2014Review
- Regulation of epithelial morphogenesis by the G protein-coupled receptor mist and its ligand fog.Science signaling · 2013Article
- Rho GTPase controls Drosophila salivary gland lumen size through regulation of the actin cytoskeleton and Moesin.Development (Cambridge, England) · 2011Article
- Article
- Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis.Developmental biology · 2010Article
- Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.The Journal of cell biology · 2010Article
- A second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila.PloS one · 2009Article
- Mechanical control of tissue morphogenesis.Circulation research · 2008Review
- Actomyosin purse strings: renewable resources that make morphogenesis robust and resilient.HFSP journal · 2008Article
- LARG and mDia1 link Galpha12/13 to cell polarity and microtubule dynamics.Molecular biology of the cell · 2008Article
- The Rap GTPase activator Drosophila PDZ-GEF regulates cell shape in epithelial migration and morphogenesis.Molecular and cellular biology · 2007Article
- Sometimes the result is not the answer: the truths and the lies that come from using the complementation test.Genetics · 2006Review
- Drosophila Incenp is required for cytokinesis and asymmetric cell division during development of the nervous system.Journal of cell science · 2006Article
- Mutational analysis of Stubble-stubbloid gene structure and function in Drosophila leg and bristle morphogenesis.Genetics · 2006Article
- DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo.The Journal of cell biology · 2005Article
- Article
- Genetic modifier screens in Drosophila demonstrate a role for Rho1 signaling in ecdysone-triggered imaginal disc morphogenesis.Genetics · 2003Article
- Roles of myosin phosphatase during Drosophila development.Development (Cambridge, England) · 2003Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
A dynamic actomyosin cytoskeleton drives many morphogenetic events. Conventional nonmuscle myosin-II (myosin) is a key chemomechanical motor that drives contraction of the actin cytoskeleton. We have explored the regulation of myosin activity by performing genetic screens to identify gene products that collaborate with myosin during Drosophila morphogenesis. Specifically, we screened for second-site noncomplementors of a mutation in the zipper gene that encodes the nonmuscle myosin-II heavy chain. We determined that a single missense mutation in the zipper(Ebr) allele gives rise to its sensitivity to second-site noncomplementation. We then identify the Rho signal transduction pathway as necessary for proper myosin function. First we show that a lethal P-element insertion interacts genetically with zipper. Subsequently we show that this second-site noncomplementing mutation disrupts the RhoGEF2 locus. Next, we show that two EMS-induced mutations, previously shown to interact genetically with zipper(Ebr), disrupt the RhoA locus. Further, we have identified their molecular lesions and determined that disruption of the carboxyl-terminal CaaX box gives rise to their mutant phenotype. Finally, we show that RhoA mutations themselves can be utilized in genetic screens. Biochemical and cell culture analyses suggest that Rho signal transduction regulates the activity of myosin. Our studies provide direct genetic proof of the biological relevance of regulation of myosin by Rho signal transduction in an intact metazoan.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.