ArticleGenetics2000
The cloning and molecular analysis of pawn-B in Paramecium tetraurelia.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Micromanipulation in Paramecium: From non-mendelian inheritance to the outlook for versatile micromachines.The Journal of eukaryotic microbiology · 2022Review
- Review
- Voltage-gated calcium channels of Paramecium cilia.The Journal of experimental biology · 2016Article
- The Paramecium germline genome provides a niche for intragenic parasitic DNA: evolutionary dynamics of internal eliminated sequences.PLoS genetics · 2012Article
- Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway.International journal of evolutionary biology · 2012Article
- Distinct subcellular localization of a group of synaptobrevin-like SNAREs in Paramecium tetraurelia and effects of silencing SNARE-specific chaperone NSF.Eukaryotic cell · 2010Article
- Ion channels in microbes.Physiological reviews · 2008Review
- Developmentally programmed DNA splicing in Paramecium reveals short-distance crosstalk between DNA cleavage sites.Nucleic acids research · 2008Article
- Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: a somatic view of the germline.Genome research · 2008Article
- Non-Mendelian inheritance induced by gene amplification in the germ nucleus of Paramecium tetraurelia.Genetics · 2005Article
- Developmentally regulated chromosome fragmentation linked to imprecise elimination of repeated sequences in paramecia.Eukaryotic cell · 2003Article
- PAK paradox: Paramecium appears to have more K(+)-channel genes than humans.Eukaryotic cell · 2003Article
- An exchanger-like protein underlies the large Mg2+ current in Paramecium.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Random sequencing of Paramecium somatic DNA.Eukaryotic cell · 2002Article
- K(+)-channel transgenes reduce K(+) currents in Paramecium, probably by a post-translational mechanism.Genetics · 2001Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Pawn mutants of Paramecium tetraurelia lack a depolarization-activated Ca(2+) current and do not swim backward. Using the method of microinjection and sorting a genomic library, we have cloned a DNA fragment that complements pawn-B (pwB/pwB). The minimal complementing fragment is a 798-bp open reading frame (ORF) that restores the Ca(2+) current and the backward swimming when expressed. This ORF contains a 29-bp intron and is transcribed and translated. The translated product has two putative transmembrane domains but no clear matches in current databases. Mutations in the available pwB alleles were found within this ORF. The d4-95 and d4-96 alleles are single base substitutions, while d4-662 (previously pawn-D) harbors a 44-bp insertion that matches an internal eliminated sequence (IES) found in the wild-type germline DNA except for a single C-to-T transition. Northern hybridizations and RT-PCR indicate that d4-662 transcripts are rapidly degraded or not produced. A second 155-bp IES in the wild-type germline ORF excises at two alternative sites spanning three asparagine codons. The pwB ORF appears to be separated from a 5' neighboring ORF by only 36 bp. The close proximity of the two ORFs and the location of the pwB protein as indicated by GFP-fusion constructs are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.