ArticleGenetics2000
MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 81 citations in OpenAlex.
- Widespread atypical UV-induced mutations form in single-stranded DNA.Science advances · 2026Article
- Article
- A genome-wide screen identifies genes that suppress the accumulation of spontaneous mutations in young and aged yeast cells.Aging cell · 2020Article
- Review
- Esc2 promotes telomere stability in response to DNA replication stress.Nucleic acids research · 2019Article
- Helicase Mechanisms During Homologous Recombination inAnnual review of biophysics · 2019Review
- Article
- Bloom syndrome helicase in meiosis: Pro-crossover functions of an anti-crossover protein.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase.Genetics · 2016Article
- Biochemical Activities and Genetic Functions of the Drosophila melanogaster Fancm Helicase in DNA Repair.Genetics · 2016Article
- Article
- Binding of the Fkh1 Forkhead Associated Domain to a Phosphopeptide within the Mph1 DNA Helicase Regulates Mating-Type Switching in Budding Yeast.PLoS genetics · 2016Article
- MTE1 Functions with MPH1 in Double-Strand Break Repair.Genetics · 2016Article
- Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance.Genes & development · 2016Article
- Differential regulation of the anti-crossover and replication fork regression activities of Mph1 by Mte1.Genes & development · 2016Article
- Article
- Functions and regulation of the multitasking FANCM family of DNA motor proteins.Genes & development · 2015Review
- The Arabidopsis thaliana homolog of the helicase RTEL1 plays multiple roles in preserving genome stability.The Plant cell · 2014Article
- Regulation of DNA pairing in homologous recombination.Cold Spring Harbor perspectives in biology · 2014Review
- FANCM-associated proteins MHF1 and MHF2, but not the other Fanconi anemia factors, limit meiotic crossovers.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the MPH1 gene from Saccharomyces cerevisiae. mph1 mutants display a spontaneous mutator phenotype. Homologs were found in archaea and in the EST libraries of Drosophila, mouse, and man. Mph1 carries the signature motifs of the DEAH family of helicases. Selected motifs were shown to be necessary for MPH1 function by introducing missense mutations. Possible indirect effects on translation and splicing were excluded by demonstrating nuclear localization of the protein and splicing proficiency of the mutant. A mutation spectrum did not show any conspicuous deviations from wild type except for an underrepresentation of frameshift mutations. The mutator phenotype was dependent on REV3 and RAD6. The mutant was sensitive to MMS, EMS, 4-NQO, and camptothecin, but not to UV light and X rays. Epistasis analyses were carried out with representative mutants from various repair pathways (msh6, mag1, apn1, rad14, rad52, rad6, mms2, and rev3). No epistatic interactions were found, either for the spontaneous mutator phenotype or for MMS, EMS, and 4-NQO sensitivity. mph1 slightly increased the UV sensitivity of mms2, rad6, and rad14 mutants, but no effect on X-ray sensitivity was observed. These data suggest that MPH1 is not part of a hitherto known repair pathway. Possible functions are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.