ArticleThe EMBO journal2000
Efficient intracellular retrotransposition of an exogenous primate retrovirus genome.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Adenovirus vectors in hematopoietic stem cell genome editing.FEBS letters · 2019Review
- Review
- Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration.PLoS pathogens · 2016Article
- The cooperative function of arginine residues in the Prototype Foamy Virus Gag C-terminus mediates viral and cellular RNA encapsidation.Retrovirology · 2014Article
- N-Myc interactor inhibits prototype foamy virus by sequestering viral Tas protein in the cytoplasm.Journal of virology · 2014Article
- Review
- The foamy virus Gag proteins: what makes them different?Viruses · 2013Review
- Innate sensing of foamy viruses by human hematopoietic cells.Journal of virology · 2012Article
- Review
- Nonintegrating foamy virus vectors.Journal of virology · 2010Article
- Molecular biology of foamy viruses.Medical microbiology and immunology · 2010Review
- Functional and structural characterization of the integrase from the prototype foamy virus.Nucleic acids research · 2009Article
- Anti-viral RNA silencing: do we look like plants?Retrovirology · 2006Review
- Restriction of foamy viruses by APOBEC cytidine deaminases.Journal of virology · 2006Article
- N-terminal Gag domain required for foamy virus particle assembly and export.Journal of virology · 2005Article
- Determination of the relative amounts of Gag and Pol proteins in foamy virus particles.Retrovirology · 2005Article
- RNA and protein requirements for incorporation of the Pol protein into foamy virus particles.Journal of virology · 2005Article
- Foamy virus--adenovirus hybrid vectors.Gene therapy · 2004Article
- Foamy virus integration.Journal of virology · 2004Article
- Retrotransposition and cell-to-cell transfer of foamy viruses.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The foamy virus (FV) subgroup of Retroviridae reverse transcribe their RNA (pre-)genome late in the replication cycle before leaving an infected cell. We studied whether a marker gene-transducing FV vector is able to shuttle to the nucleus and integrate into host cell genomic DNA. While a potential intracellular retrotransposition of vectors derived from other retroviruses was below the detection limit of our assay, we found that up to 5% of cells transfected with the FV vector were stably transduced, harboring 1 to approximately 10 vector integrants. Generation of the integrants depended on expression of functional capsid, reverse transcriptase and integrase proteins, and did not involve an extracellular step. PCR analysis of the U3 region of the 5' long terminal repeat and determination of proviral integration sites showed that a reverse transcription step had taken place to generate the integrants. Co-expression of a mutated envelope allowing particle egress and avoiding extracellular infection resulted in a significantly increased rescue of cells harboring integrants, suggesting that accumulation of proviruses via intracellular retrotransposition represents an integral part of the FV replication strategy.
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Registered trials
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