ArticleThe EMBO journal2000
Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 72 citations in OpenAlex.
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- From protein structure to function via single crystal optical spectroscopy.Frontiers in molecular biosciences · 2015Review
- Disturbed local auxin homeostasis enhances cellular anisotropy and reveals alternative wiring of auxin-ethylene crosstalk in Brachypodium distachyon seminal roots.PLoS genetics · 2013Article
- A novel 3-sulfinopropionyl coenzyme A (3SP-CoA) desulfinase from Advenella mimigardefordensis strain DPN7T acting as a key enzyme during catabolism of 3,3'-dithiodipropionic acid is a member of the acyl-CoA dehydrogenase superfamily.Journal of bacteriology · 2013Article
- Characterization of C-S Lyase from C. diphtheriae: a possible target for new antimicrobial drugs.BioMed research international · 2013Article
- Identification of novel inhibitors of 1-aminocyclopropane-1-carboxylic acid synthase by chemical screening in Arabidopsis thaliana.The Journal of biological chemistry · 2010Article
- Crystallization and preliminary X-ray analysis of betaC-S lyases from two oral Streptococci.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- A 52-kDa leucyl aminopeptidase from treponema denticola is a cysteinylglycinase that mediates the second step of glutathione metabolism.The Journal of biological chemistry · 2008Article
- α-Vinylic Amino Acids: Occurrence, Asymmetric Synthesis and Biochemical Mechanisms.Tetrahedron, asymmetry · 2006Article
- Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage.Protein science : a publication of the Protein Society · 2005Article
- Enzyme adaptation to alkaline pH: atomic resolution (1.08 A) structure of phosphoserine aminotransferase from Bacillus alcalophilus.Protein science : a publication of the Protein Society · 2005Article
- Evolutionarily conserved regions and hydrophobic contacts at the superfamily level: The case of the fold-type I, pyridoxal-5'-phosphate-dependent enzymes.Protein science : a publication of the Protein Society · 2004Article
- A genomic overview of pyridoxal-phosphate-dependent enzymes.EMBO reports · 2003Review
- Treponema denticola cystalysin exhibits significant alanine racemase activity accompanied by transamination: mechanistic implications.The Biochemical journal · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cystalysin is a C(beta)-S(gamma) lyase from the oral pathogen Treponema denticola catabolyzing L-cysteine to produce pyruvate, ammonia and H(2)S. With its ability to induce cell lysis, cystalysin represents a new class of pyridoxal 5'-phosphate (PLP)-dependent virulence factors. The crystal structure of cystalysin was solved at 1.9 A resolution and revealed a folding and quaternary arrangement similar to aminotransferases. Based on the active site architecture, a detailed catalytic mechanism is proposed for the catabolism of S-containing amino acid substrates yielding H(2)S and cysteine persulfide. Since no homologies were observed with known haemolysins the cytotoxicity of cystalysin is attributed to this chemical reaction. Analysis of the cystalysin-L-aminoethoxyvinylglycine (AVG) complex revealed a 'dead end' ketimine PLP derivative, resulting in a total loss of enzyme activity. Cystalysin represents an essential factor of adult periodontitis, therefore the structure of the cystalysin-AVG complex may provide the chemical basis for rational drug design.
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