ArticleThe Journal of clinical investigation2000
Heat shock protein 70 prevents secretagogue-induced cell injury in the pancreas by preventing intracellular trypsinogen activation.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 84 citations in OpenAlex.
- The Foundation for Engineering a Pancreatic Islet Niche.Frontiers in endocrinology · 2022Review
- Article
- Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology.Nature protocols · 2014Article
- The role of protein synthesis and digestive enzymes in acinar cell injury.Nature reviews. Gastroenterology & hepatology · 2013Review
- Prior peritoneal lavage with hot 0.9 % saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in rats.Molecular biology reports · 2013Article
- Evidence of multimeric forms of HSP70 with phosphorylation on serine and tyrosine residues--implications for roles of HSP70 in detection of GI cancers.Asian Pacific journal of cancer prevention : APJCP · 2013Article
- Mouse pancreas tissue slice culture facilitates long-term studies of exocrine and endocrine cell physiology in situ.PloS one · 2013Article
- Article
- Heat shock proteins and autophagy in rats with cerulein-induced acute pancreatitis.Gut and liver · 2011Article
- Down-regulation of HSP60 expression by RNAi increases lipopolysaccharide- and cerulein-induced damages on isolated rat pancreatic tissues.Cell stress & chaperones · 2010Article
- Ascitic fluid and serum from rats with acute pancreatitis injure rat pancreatic tissues and alter the expression of heat shock protein 60.Cell stress & chaperones · 2010Article
- Article
- Beer and its non-alcoholic compounds: role in pancreatic exocrine secretion, alcoholic pancreatitis and pancreatic carcinoma.International journal of environmental research and public health · 2010Review
- Protease activation during in vivo pancreatitis is dependent on calcineurin activation.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Reducing extracellular pH sensitizes the acinar cell to secretagogue-induced pancreatitis responses in rats.Gastroenterology · 2009Article
- Tissue-specific regulation and expression of heat shock proteins in type 2 diabetic monkeys.Cell stress & chaperones · 2009Article
- Functional role of J domain of cysteine string protein in Ca2+-dependent secretion from acinar cells.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Effect of non-alcoholic compounds of alcoholic drinks on the pancreas.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] · 2007Review
- In vitro evidence for role of ERK, p38, and JNK in exocrine pancreatic cytokine production.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2006Article
- Increased heat shock protein 70 expression in the pancreas of rats with endotoxic shock.World journal of gastroenterology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Rodents given a supramaximally stimulating dose of cholecystokinin or its analogue cerulein develop acute pancreatitis with acinar cell injury, pancreatic inflammation, and intrapancreatic digestive enzyme (i.e., trypsinogen) activation. Prior thermal stress is associated with heat shock protein 70 (HSP70) expression and protection against cerulein-induced pancreatitis. However, thermal stress can also induce expression of other HSPs. The current studies were performed using an in vitro system to determine whether HSP70 can actually mediate protection against pancreatitis and, if so, to define the mechanism underlying that protection. We show that in vitro exposure of freshly prepared rat pancreas fragments to a supramaximally stimulating dose of cerulein results in changes similar to those noted in cerulein-induced pancreatitis, i.e., intra-acinar cell trypsinogen activation and acinar cell injury. Short-term culture of the fragments results in HSP70 expression and loss of the pancreatitis-like changes noted after addition of cerulein. The culture-induced enhanced HSP70 expression can be prevented by addition of either the flavonoid antioxidant quercetin or an antisense oligonucleotide to HSP70. Under these latter conditions, addition of a supramaximally stimulating concentration of cerulein results in trypsinogen activation and acinar cell injury. These findings indicate that the protection against cerulein-induced pancreatitis that follows culture-induced (and possibly thermal) stress is mediated by HSP70. They suggest that the HSP acts by preventing trypsinogen activation within acinar cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.