ArticleThe Journal of physiology2000
Cloning and expression of a FMRFamide-gated Na(+) channel from Helisoma trivolvis and comparison with the native neuronal channel.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Flipping the switch: Illuminating inverted ligand activation of peptide-gated ion channels.Biophysical journal · 2025Article
- Structure and mechanism of a neuropeptide-activated channel in the ENaC/DEG superfamily.Nature chemical biology · 2023Article
- Aromatic amino acids in the finger domain of the FMRFamide-gated NaPflugers Archiv : European journal of physiology · 2023Article
- The FMRF-NH2 gated sodium channel of Biomphalaria glabrata: Localization and expression following infection by Schistosoma mansoni.PLoS neglected tropical diseases · 2023Article
- Acid-sensing ion channels in sensory signaling.American journal of physiology. Renal physiology · 2020Review
- A NaThe Journal of biological chemistry · 2019Article
- New techniques, applications and perspectives in neuropeptide research.The Journal of experimental biology · 2018Review
- The nonproton ligand of acid-sensing ion channel 3 activates mollusk-specific FaNaC channels via a mechanism independent of the native FMRFamide peptide.The Journal of biological chemistry · 2017Article
- Modulation of the FMRFamide-gated NaPflugers Archiv : European journal of physiology · 2017Article
- Proton and non-proton activation of ASIC channels.PloS one · 2017Article
- Exploration of the Peptide Recognition of an Amiloride-sensitive FMRFamide Peptide-gated Sodium Channel.The Journal of biological chemistry · 2016Article
- Diversity of the RFamide Peptide Family in Mollusks.Frontiers in endocrinology · 2014Review
- Parasite neuropeptide biology: Seeding rational drug target selection?International journal for parasitology. Drugs and drug resistance · 2012Review
- The neuronal control of cardiac functions in Molluscs.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2011Review
- Three homologous subunits form a high affinity peptide-gated ion channel in Hydra.The Journal of biological chemistry · 2010Article
- Heterologous expression of the invertebrate FMRFamide-gated sodium channel as a mechanism to selectively activate mammalian neurons.Neuroscience · 2008Article
- Gene expression profile of Clonorchis sinensis metacercariae.Parasitology research · 2008Article
- Molecular cloning and functional characterization of the Aplysia FMRFamide-gated Na+ channel.Pflugers Archiv : European journal of physiology · 2006Article
- Domain near TM1 influences agonist and antagonist responses of peptide-gated Na+ channels.Pflugers Archiv : European journal of physiology · 2005Article
- Identification in Drosophila melanogaster of the invertebrate G protein-coupled FMRFamide receptor.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
We have cloned a cDNA encoding a Phe-Met-Arg-Phe-NH(2) (FMRFamide)-gated Na(+) channel from nervous tissue of the pond snail Helisoma trivolvis (HtFaNaC) and expressed the channel in Xenopus oocytes. The deduced amino acid sequence of the protein expressed by HtFaNaC is 65 % identical to that of the FMRFamide-gated channel cloned from Helix aspersa (HaFaNaC). HtFaNaC expressed in oocytes was less sensitive to FMRFamide (EC(50) = 70 microM) than HaFaNaC (EC(50) = 2 microM). The two had a similar selectivity for Na+. The amplitude of the FMRFamide response of HtFaNaC was increased by reducing the extracellular concentration of divalent cations. The conductance of the two channels was similar, but the mean open time of unitary events was shorter for expressed HtFaNaC compared to expressed HaFaNaC. Each channel was susceptible to peptide block by high agonist concentrations. In marked contrast to HaFaNaC and other amiloride-sensitive Na(+) channels, amiloride, and the related drugs benzamil and 5-(N-ethyl-N-isopropyl)-amiloride (EIPA), enhanced the FMRFamide response in oocytes expressing HtFaNaC cRNA. The potentiating effects of EIPA and benzamil were greater than those of amiloride. Unitary current analysis showed that with such drugs, there was channel blockade as well as an increased probability of channel opening. The similar permeability of the oocyte-expressed HtFaNaC and the Helisoma neuronal channel, and the susceptibility of both to agonist blockade and blockade by divalent cations, suggest that the channels are the same. However, neuronal channels were less susceptible to enhancement by amiloride analogues and in some patches were more sensitive to FMRFamide than expressed HtFaNaC.
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