ArticleJournal of clinical microbiology2000
Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- New gene markers for classification and quantification of Faecalibacterium spp. in the human gut.FEMS microbiology ecology · 2023Article
- Complete mitochondrial genome of Asian longhorned tick,Mitochondrial DNA. Part B, Resources · 2021Article
- Screening forPathogens (Basel, Switzerland) · 2020Article
- Article
- Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Development of a PCR Assay for Diagnosing Trematode (Opisthorchis and Haplorchis) Infections in Human Stools.The American journal of tropical medicine and hygiene · 2017Article
- Ixodes holocyclus Tick-Transmitted Human Pathogens in North-Eastern New South Wales, Australia.Tropical medicine and infectious disease · 2016Article
- A Novel Isothermal Assay of Borrelia burgdorferi by Recombinase Polymerase Amplification with Lateral Flow Detection.International journal of molecular sciences · 2016Article
- Detection of microbial agents in ticks collected from migratory birds in central Italy.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2014Article
- Phenotypic and genotypic analysis of Borrelia spp. isolated from Ixodes ricinus ticks by using electrophoretic chips and real-time polymerase chain reaction.Folia microbiologica · 2007Article
- Prevalence of Borrelia burgdorferi sensu lato in the tick Ixodes ricinus in the Styrian mountains of Austria.Wiener klinische Wochenschrift · 2006Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Diagnosis of lyme borreliosis.Clinical microbiology reviews · 2005Review
- Quantitative analysis of Borrelia burgdorferi gene expression in naturally (tick) infected mouse strains.Medical microbiology and immunology · 2005Article
- Rapid detection of Wuchereria bancrofti in mosquitoes by LightCycler polymerase chain reaction and melting curve analysis.Parasitology research · 2004Article
- Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.Methods in microbiology · 2004Review
- Real-time PCR for simultaneous detection and quantification of Borrelia burgdorferi in field-collected Ixodes scapularis ticks from the Northeastern United States.Applied and environmental microbiology · 2003Article
- Real-time PCR for detection and quantitation of leishmania in mouse tissues.Journal of clinical microbiology · 2002Article
- Quantitative detection of Borrelia burgdorferi in 2-millimeter skin samples of erythema migrans lesions: correlation of results with clinical and laboratory findings.Journal of clinical microbiology · 2002Article
- Distribution of clinically relevant Borrelia genospecies in ticks assessed by a novel, single-run, real-time PCR.Journal of clinical microbiology · 2002Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplified product. A total of 32 DNA samples were tested. Of them three were isolated from B. burgdorferi reference strains and 16 were isolated from B. burgdorferi strains cultured from Ixodes ricinus ticks; 13 were directly isolated from nine human biopsy specimens and four I. ricinus tick midguts. The melting temperature of B. garinii was 2 degrees C lower than that of B. burgdorferi sensu stricto and B. afzelii. Melting curve analysis offers a rapid alternative for identification and detection of B. burgdorferi sensu lato genospecies.
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