ArticleJournal of clinical microbiology2000
Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 3 of them syntheses that pooled it.
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Who cites it
30 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- Molecular assays for the diagnosis of sepsis in neonates.The Cochrane database of systematic reviews · 2017Pooled it
- Pooled it
- Prolonged Post-Discontinuation Antibiotic Exposure in Very Low Birth Weight Neonates at Risk for Early-Onset Sepsis.Journal of the Pediatric Infectious Diseases Society · 2021Article
- Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis.Critical care (London, England) · 2018Article
- Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples.European journal of pediatrics · 2018Article
- Escherichia coli early-onset sepsis: trends over two decades.European journal of pediatrics · 2017Article
- Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for rapid diagnosis of neonatal sepsis.The Indian journal of medical research · 2016Article
- Point of care investigations in pediatric care to improve health care in rural areas.Indian journal of pediatrics · 2013Article
- Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.PloS one · 2013Article
- Development of PCR-based method for detection of Enterobacteriaceae in septicemia.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2012Article
- A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.Journal of clinical microbiology · 2011Article
- Molecular microbiological methods in the diagnosis of neonatal sepsis.Expert review of anti-infective therapy · 2010Review
- Efficacy of bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR for detecting neonatal sepsis: a case control study.BMC pediatrics · 2010Article
- Procession to pediatric bacteremia and sepsis: covert operations and failures in diplomacy.Pediatrics · 2010Review
- New approaches to preventing, diagnosing, and treating neonatal sepsis.PLoS medicine · 2010Article
- PCR-based diagnosis of human fungal infections.Expert review of anti-infective therapy · 2009Review
- Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis.Journal of clinical microbiology · 2009Article
- Comparison of broad range 16S rDNA PCR and conventional blood culture for diagnosis of sepsis in the newborn: a case control study.BMC pediatrics · 2009Article
- A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples.Medical microbiology and immunology · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Ten percent of infants born in the United States are admitted to neonatal intensive care units (NICU) annually. Approximately one-half of these admissions are from term infants (>34 weeks of gestation) at risk for systemic infection. Most of the term infants are not infected but rather have symptoms consistent with other medical conditions that mimic sepsis. The current standard of care for evaluating bacterial sepsis in the newborn is performing blood culturing and providing antibiotic therapy while awaiting the 48-h preliminary result of culture. Implementing a more rapid means of ruling out sepsis in term newborns could result in shorter NICU stays and less antibiotic usage. The purpose of this feasibility study was to compare the utility of PCR to that of conventional culture. To this end, a total of 548 paired blood samples collected from infants admitted to the NICU for suspected sepsis were analyzed for bacterial growth using the BACTEC 9240 instrument and for the bacterial 16S rRNA gene using a PCR assay which included a 5-h preamplification culturing step. The positivity rates by culture and PCR were 25 (4.6%) and 27 (4.9%) positive specimens out of a total of 548 specimens, respectively. The comparison revealed sensitivity, specificity, and positive and negative predictive values of 96.0, 99. 4, 88.9, and 99.8%, respectively, for PCR. In summary, this PCR-based approach, requiring as little as 9 h of turnaround time and blood volumes as small as 200 microl, correlated well with conventional blood culture results obtained for neonates suspected of having bacterial sepsis.
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