ArticleJournal of clinical microbiology2000
Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed, 214 citations in OpenAlex.
- Article
- Automated web-based typing of Clostridioides difficile ribotypes via MALDI-TOF MS.BMC bioinformatics · 2025Article
- Ribotypes and New Virulent Strains Across Europe.Advances in experimental medicine and biology · 2024Review
- High Prevalence ofPathogens (Basel, Switzerland) · 2023Article
- Characterization ofMicroorganisms · 2022Article
- Real-life experience with fidaxomicin in Clostridioides difficile infection: a multicentre cohort study on 244 episodes.Infection · 2021Article
- Rapid Classification ofMicroorganisms · 2021Article
- Simultaneous detection and ribotyping of Clostridioides difficile, and toxin gene detection directly on fecal samples.Antimicrobial resistance and infection control · 2021Article
- Recurrent community-acquired Clostridium(Clostridioides)difficile infection in Serbianchildren.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Clostridium difficile in wild rodents and insectivores in the Netherlands.Letters in applied microbiology · 2019Article
- Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present.Microorganisms · 2019Review
- Whole-genome sequencing reveals nosocomial Clostridioides difficile transmission and a previously unsuspected epidemic scenario.Scientific reports · 2019Article
- Genome Location Dictates the Transcriptional Response to PolC Inhibition inAntimicrobial agents and chemotherapy · 2019Article
- Variable spectrum of disease and risk factors of peripartum Clostridium difficile infection: report of 14 cases from French hospitals and literature review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Review
- Article
- Article
- Epidemiology ofThe open microbiology journal · 2017Article
- Genetic Diversity and Evidence for Transmission of Streptococcus mutans by DiversiLab rep-PCR.Journal of microbiological methods · 2016Article
- Development and evaluation of double locus sequence typing for molecular epidemiological investigations of Clostridium difficile.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Analysis of TcdB Proteins within the Hypervirulent Clade 2 Reveals an Impact of RhoA Glucosylation on Clostridium difficile Proinflammatory Activities.Infection and immunity · 2016Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. However, the comparative typing ability, reproducibility, discriminatory power, and efficiency of these methods have not been fully investigated. We compared the results of three methods-AP-PCR with three different primers (AP3, AP4, and AP5), PCR-ribotyping, and PFGE (with SmaI endonuclease)-to differentiate 99 strains of C. difficile that had been previously serogrouped. Typing abilities were 100% for PCR-ribotyping and AP-PCR with AP3 and 90% for PFGE, due to early DNA degradation in strains from serogroup G. Reproducibilities were 100% for PCR-ribotyping and PFGE but only 88% for AP-PCR with AP3, 67% for AP-PCR with AP4, and 33% for AP-PCR with AP5. Discriminatory power for unrelated strains was >0.95 for all the methods but was lower for PCR-ribotyping among serogroups D and C. PCR-based methods were easier and quicker to perform, but their fingerprints were more difficult to interpret than those of PFGE. We conclude that PCR-ribotyping offers the best combination of advantages as an initial typing tool for C. difficile.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.