ReviewPhilosophical transactions of the Royal Society of London. Series B, Biological sciences2000
Measurement of bacterial gene expression in vivo.
Review in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Identifying Bacterial Lineages in Salmonella by Flow Cytometry.EcoSal Plus · 2022Review
- An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.Cellular microbiology · 2019Article
- The Bacillus BioBrick Box 2.0: expanding the genetic toolbox for the standardized work with Bacillus subtilis.Scientific reports · 2017Article
- Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP.Pathogens (Basel, Switzerland) · 2016Article
- The EPA2 adhesin encoding gene is responsive to oxidative stress in the opportunistic fungal pathogen Candida glabrata.Current genetics · 2015Article
- 16S rRNA and As-Related Functional Diversity: Contrasting Fingerprints in Arsenic-Rich Sediments from an Acid Mine Drainage.Microbial ecology · 2015Article
- Clinical outcomes of type III Pseudomonas aeruginosa bacteremia.Critical care medicine · 2012Article
- Comparison and calibration of different reporters for quantitative analysis of gene expression.Biophysical journal · 2011Article
- Pneumococcal virulence gene expression and host cytokine profiles during pathogenesis of invasive disease.Infection and immunity · 2008Article
- Identification of group A Streptococcus antigenic determinants upregulated in vivo.Infection and immunity · 2005Article
- Unraveling the secret lives of bacteria: use of in vivo expression technology and differential fluorescence induction promoter traps as tools for exploring niche-specific gene expression.Microbiology and molecular biology reviews : MMBR · 2005Review
- Single-copy green fluorescent protein gene fusions allow accurate measurement of Salmonella gene expression in vitro and during infection of mammalian cells.Applied and environmental microbiology · 2003Article
- Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients.Infection and immunity · 2003Article
- Analysis of light and CO(2) regulation in Chlamydomonas reinhardtii using genome-wide approaches.Photosynthesis research · 2003Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complexities of bacterial gene expression during mammalian infection cannot be addressed by in vitro experiments. We know that the infected host represents a complex and dynamic environment, which is modified during the infection process, presenting a variety of stimuli to which the pathogen must respond if it is to be successful. This response involves hundreds of ivi (in vivo-induced) genes which have recently been identified in animal and cell culture models using a variety of technologies including in vivo expression technology, differential fluorescence induction, subtractive hybridization and differential display. Proteomic analysis is beginning to be used to identify IVI proteins, and has benefited from the availability of genome sequences for increasing numbers of bacterial pathogens. The patterns of bacterial gene expression during infection remain to be investigated. Are ivi genes expressed in an organ-specific or cell-type-specific fashion? New approaches are required to answer these questions. The uses of the immunologically based in vivo antigen technology system, in situ PCR and DNA microarray analysis are considered. This review considers existing methods for examining bacterial gene expression in vivo, and describes emerging approaches that should further our understanding in the future.
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Registered trials
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