ArticleGene therapy2000
An improved anion-exchange HPLC method for the detection and purification of adenoviral particles.
Article in Gene therapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- Authorizing of Immunogenicity of Concentrated and Purified Newcastle Disease Virus V4 Strain using Downstream Processing.Archives of Razi Institute · 2024Article
- Evolving Horizons: Adenovirus Vectors' Timeless Influence on Cancer, Gene Therapy and Vaccines.Viruses · 2023Review
- Chromatographic Purification of Viral Vectors for Gene Therapy Applications.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Adenoviral vectors for cardiovascular gene therapy applications: a clinical and industry perspective.Journal of molecular medicine (Berlin, Germany) · 2022Review
- Identification of Recombinant Chimpanzee Adenovirus C68 Degradation Products Detected by AEX-HPLC.Frontiers in bioengineering and biotechnology · 2022Article
- Aluminum particles generated during millisecond electric pulse application enhance adenovirus-mediated gene transfer in L929 cells.Scientific reports · 2021Article
- Characterization of Recombinant Chimpanzee Adenovirus C68 Low and High-Density Particles: Impact on Determination of Viral Particle Titer.Frontiers in bioengineering and biotechnology · 2021Article
- Article
- Standard free droplet digital polymerase chain reaction as a new tool for the quality control of high-capacity adenoviral vectors in small-scale preparations.Human gene therapy methods · 2015Article
- Downstream processing and chromatography based analytical methods for production of vaccines, gene therapy vectors, and bacteriophages.Human vaccines & immunotherapeutics · 2015Review
- The FANC pathway is activated by adenovirus infection and promotes viral replication-dependent recombination.Nucleic acids research · 2011Article
- Purification of Bionanoparticles.Chemical engineering & technology · 2008Article
- Does neuronal expression of GDNF effectively protect dopaminergic neurons in a rat model of Parkinson's disease?Gene therapy · 2007Article
- High-performance liquid chromatography method for rapid assessment of viral particle number in crude adenoviral lysates of mixed serotype.Gene therapy · 2007Article
- Article
- A rapid and efficient method for purification of recombinant adenovirus with arginine-glycine-aspartic acid-modified fibers.Analytical biochemistry · 2006Article
- Increased plasma levels of LDL cholesterol in rabbits after adenoviral overexpression of human scavenger receptor class B type I.Journal of molecular medicine (Berlin, Germany) · 2005Article
- Purification of adenovirus and adeno-associated virus: comparison of novel membrane-based technology to conventional techniques.Gene therapy · 2005Article
- Downstream processing of viral vectors and vaccines.Gene therapy · 2005Review
- Chromatographic purification of recombinant adenoviral and adeno-associated viral vectors: methods and implications.Gene therapy · 2005Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an anion-exchange high-performance liquid chromatography (HPLC) method using Q Sepharose XL (Amersham Pharmacia Biotech) as adsorbent to analyze samples containing adenovirus. This method has several major advantages over the HPLC method previously described for quantitating particles, namely (1) a >10-fold improvement in the detection limit of adenovirus in crude preparations; (2) absence of interferences originating from nucleic acids and proteins which usually contaminate crude samples; (3) unprecedented sharpness and symmetry of adenovirus peak, rendering the identification of the viral peak unambiguous, even in extremely crude and dilute preparations; and (4) no enzymatic treatment required even for crude samples. This assay was used to quantitate particles in samples taken at the transfection and amplification stages of production of various recombinant adenovirus, and in cultures of wild-type adenovirus of different serotypes. A modification of this analytical method was also developed for the purification of infectious adenovirus particles, including fiber-modified and third-generation recombinant viruses, giving highly purified preparations from low-titer crude lysates with an excellent overall recovery (50-74%).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.