ArticleNucleic acids research2000
The role of region II in the RNA polymerase sigma factor sigma(N) (sigma(54)).
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Alternative Sigma Factors of RNA Polymerase as Master Regulators in the Pathogenic SpirochaetePathogens (Basel, Switzerland) · 2025Review
- Transcriptional regulation of soluble methane monooxygenase via enhancer-binding protein derived fromApplied and environmental microbiology · 2023Article
- Mechanisms of σJournal of molecular biology · 2019Review
- Defining the Metabolic Functions and Roles in Virulence of the rpoN1 and rpoN2 Genes in Ralstonia solanacearum GMI1000.PloS one · 2015Article
- The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.Microbiology and molecular biology reviews : MMBR · 2012Review
- Construction and functional analyses of a comprehensive sigma54 site-directed mutant library using alanine-cysteine mutagenesis.Nucleic acids research · 2009Article
- Organization of an activator-bound RNA polymerase holoenzyme.Molecular cell · 2008Article
- Interactions of regulated and deregulated forms of the sigma54 holoenzyme with heteroduplex promoter DNA.Nucleic acids research · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial RNA polymerase holoenzymes containing the sigma subunit sigma(N) (sigma(54)) can form a stable closed complex with promoter DNA but only undergo transition to an open complex and transcription initiation when acted on by an activator protein. Proteins of the sigma(N) family have a conserved N-terminal region of 50 amino acids (Region I) that is separated from a conserved C-terminal region of around 360 amino acids (Region III) by a much more variable sequence of between 30 and 110 residues (Region II). We have investigated the role of Region II in Klebsiella pneumoniae sigma(N) by studying the properties of deletions of all or part of the region both in vivo and in vitro. We found that whilst Region II is not essential, deletion of all or part of it can significantly impair sigma(N) activity. Deletions have effects on DNA binding by the isolated sigma factor and on holoenzyme formation, but the most marked effects are on transition of the holoenzyme from the closed to the open complex in the presence of the activator protein.
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