ArticleNucleic acids research2000
Identification of high affinity Tbf1p-binding sites within the budding yeast genome.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Yeast heterochromatin stably silences only weak regulatory elements by altering burst duration.Cell reports · 2024Article
- Yeast Heterochromatin Only Stably Silences Weak Regulatory Elements by Altering Burst Duration.bioRxiv : the preprint server for biology · 2023Article
- Partners in crime: Tbf1 and Vid22 promote expansions of long human telomeric repeats at an interstitial chromosome position in yeast.PNAS nexus · 2022Article
- VID22 counteracts G-quadruplex-induced genome instability.Nucleic acids research · 2021Article
- Regulation of Antigenic Variation byPathogens (Basel, Switzerland) · 2021Review
- Telomeric and Sub-Telomeric Structure and Implications in Fungal Opportunistic Pathogens.Microorganisms · 2021Review
- Duplex Telomere-Binding Proteins in Fungi With Canonical Telomere Repeats: New Lessons in the Rapid Evolution of Telomere Proteins.Frontiers in genetics · 2021Review
- Structurally distinct telomere-binding proteins in Ustilago maydis execute non-overlapping functions in telomere replication, recombination, and protection.Communications biology · 2020Article
- Pif1, RPA, and FEN1 modulate the ability of DNA polymerase δ to overcome protein barriers during DNA synthesis.The Journal of biological chemistry · 2020Article
- In vivo chromatin organization on native yeast telomeric regions is independent of a cis-telomere loopback conformation.Epigenetics & chromatin · 2020Article
- Review
- Double-stranded telomeric DNA binding proteins: Diversity matters.Cell cycle (Georgetown, Tex.) · 2017Review
- Replication of telomeres and the regulation of telomerase.Cold Spring Harbor perspectives in biology · 2013Review
- Tbf1 and Vid22 promote resection and non-homologous end joining of DNA double-strand break ends.The EMBO journal · 2013Article
- Identification of telomerase RNAs from filamentous fungi reveals conservation with vertebrates and yeasts.PloS one · 2013Article
- Synergism of the two Myb domains of Tay1 protein results in high affinity binding to telomeres.The Journal of biological chemistry · 2012Article
- Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to end.Genetics · 2012Review
- Subtelomere-binding protein Tbf1 and telomere-binding protein Rap1 collaborate to inhibit localization of the Mre11 complex to DNA ends in budding yeast.Molecular biology of the cell · 2012Article
- Endogenous ribosomal frameshift signals operate as mRNA destabilizing elements through at least two molecular pathways in yeast.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast TBF1 gene is essential for mitotic growth and encodes a protein that binds the human telomere repeats in vitro, although its cellular function is unknown. The sequence of the DNA-binding domain of Tbf1p is more closely related to that of the human telomeric proteins TRF1 and TRF2 than to any yeast protein sequence, yet the functional homologue of TRF1 and TRF2 is thought to be Rap1p. In this study we show that the Tbf1p DNA-binding domain can target the Gal4 transactivation domain to a (TTAGGG)(n) sequence inserted in the yeast genome, supporting the model that Tbf1p binds this sub-telomeric repeat motif in vivo. Immunofluorescence of Tbf1p shows a spotty pattern throughout the interphase nucleus and along synapsed chromosomes in meiosis, suggesting that Tbf1p binds internal chromosomal sites in addition to sub-telomeric regions. PCR-assisted binding site selection was used to define a consensus for high affinity Tbf1p-binding sites. Compilation of 50 selected oligonucleotides identified the consensus TAGGGTTGG. Five potential Tbf1p-binding sites resulting from a search of the total yeast genome were tested directly in gel shift assays and shown to bind Tbf1p efficiently in vitro, thus confirming this as a valid consensus for Tbf1p recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.