ArticleNucleic acids research2000
A new MALDI-TOF based mini-sequencing assay for genotyping of SNPS.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- Predictors of poor response during asthma therapy differ with definition of outcome.Pharmacogenomics · 2009Trial
- Association of corticotropin-releasing hormone receptor-2 genetic variants with acute bronchodilator response in asthma.Pharmacogenetics and genomics · 2008Trial
- Polymorphisms in signal transducer and activator of transcription 3 and lung function in asthma.Respiratory research · 2005Trial
- Separate and interacting effects within the catechol-O-methyltransferase (COMT) are associated with schizophrenia.Molecular psychiatry · 2005Trial
- TBX21: a functional variant predicts improvement in asthma with the use of inhaled corticosteroids.Proceedings of the National Academy of Sciences of the United States of America · 2004Trial
- The Association between the rs6656401 Locus of the CR1 Gene and Structural Alterations of Brain Effects in Han Chinese Patients with Alzheimer's Disease.Current Alzheimer research · 2025Article
- Enzymatic Cleavage of 3'-Esterified Nucleotides Enables a Long, Continuous DNA Synthesis.Scientific reports · 2020Article
- Article
- Association Study Between Metallothionein-3 Protein Polymorphisms and Autism.Neurotoxicity research · 2018Article
- A novel genotyping method for detection of the CRHR1 (rs1396862: C>T) gene variation among North Indian population.Molecular biology reports · 2014Article
- Follow-up of a major psychosis linkage site in 13q13-q14 reveals significant association in both case-control and family samples.Biological psychiatry · 2013Article
- Effect modification of air pollution on Urinary 8-Hydroxy-2'-Deoxyguanosine by genotypes: an application of the multiple testing procedure to identify significant SNP interactions.Environmental health : a global access science source · 2010Article
- Association of the 98T ELAM-1 polymorphism with increased bleeding after cardiac surgery.Journal of cardiothoracic and vascular anesthesia · 2010Article
- Air pollution and homocysteine: more evidence that oxidative stress-related genes modify effects of particulate air pollution.Epidemiology (Cambridge, Mass.) · 2010Article
- Lipid and endothelium-related genes, ambient particulate matter, and heart rate variability--the VA Normative Aging Study.Journal of epidemiology and community health · 2010Article
- Black carbon exposure, oxidative stress genes, and blood pressure in a repeated-measures study.Environmental health perspectives · 2009Article
- Candidate genes for respiratory disease associated with markers of inflammation and endothelial dysfunction in elderly men.Atherosclerosis · 2009Article
- Simple, efficient, and cost-effective multiplex genotyping with matrix assisted laser desorption/ionization time-of-flight mass spectrometry of hemoglobin beta gene mutations.The Journal of molecular diagnostics : JMD · 2009Article
- Postural changes in blood pressure associated with interactions between candidate genes for chronic respiratory diseases and exposure to particulate matter.Environmental health perspectives · 2009Article
- IL1B polymorphisms modulate cystic fibrosis lung disease.Pediatric pulmonology · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A new MALDI-TOF based mini-sequencing assay termed VSET was developed for genotyping of SNPs. In this assay, specific fragments of genomic DNA containing the SNP site(s) are first amplified, followed by mini-sequencing in the presence of three ddNTPs and the fourth nucleotide in the deoxy form. In this way, the primer is extended by only one base from one allele, while it is typically extended by two bases from another allele. The products are then analyzed using MALDI-TOF mass spectrometry. The genotype of the SNP site is identified based on the number of nucleotides added. This assay has been examined using both synthetic and genomic DNA samples. In addition, multiplexed assays were successfully performed to genotype four SNP sites in a single tube. The main aspect of this assay is that it can overcome the key problems associated with the currently used mini-sequencing methods. First, it significantly reduces the stringent high-resolution and extensive desalting requirements that are essential to the pinpoint assay. Second, it avoids the long extension problem associated with the PROBE assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.