ArticleNucleic acids research2000
PCR-generated padlock probes detect single nucleotide variation in genomic DNA.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Programmable AND-gate strategy to reduce false positives in rolling circle amplification.Biosensors & bioelectronics · 2026Article
- Rolling Circle Amplification as a Molecular Tool for Spatially Resolved Signal Amplification in Single Molecule Counting Assay.Biosensors · 2025Review
- Detailed analysis of HTT repeat elements in human blood using targeted amplification-free long-read sequencing.Human mutation · 2018Article
- ConcatSeq: A method for increasing throughput of single molecule sequencing by concatenating short DNA fragments.Scientific reports · 2017Article
- Rolling circle amplification for direct detection of rpoB gene mutations in Mycobacterium tuberculosis isolates from clinical specimens.Journal of clinical microbiology · 2014Article
- Enzymatic production of 'monoclonal stoichiometric' single-stranded DNA oligonucleotides.Nature methods · 2013Article
- Next-generation mapping of complex traits with phenotype-based selection and introgression.Genetics · 2011Article
- Target-enrichment strategies for next-generation sequencing.Nature methods · 2010Review
- A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis.BMC biotechnology · 2007Article
- Multiplex amplification of all coding sequences within 10 cancer genes by Gene-Collector.Nucleic acids research · 2007Article
- Lock and roll: single-molecule genotyping in situ using padlock probes and rolling-circle amplification.Histochemistry and cell biology · 2006Review
- Circle-to-circle amplification for precise and sensitive DNA analysis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Parallel gene analysis with allele-specific padlock probes and tag microarrays.Nucleic acids research · 2003Article
- Real-time monitoring of rolling-circle amplification using a modified molecular beacon design.Nucleic acids research · 2002Article
- Padlock oligonucleotides as a tool for labeling superhelical DNA.Nucleic acids research · 2002Article
- Rolling-circle amplification under topological constraints.Nucleic acids research · 2002Article
- L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs).Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circularizing oligonucleotide probes, so-called padlock probes, have properties that should prove valuable in a wide range of genetic investigations, including in situ analyses, genotyping and measurement of gene expression. However, padlock probes can be difficult to obtain by standard oligonucleotide synthesis because they are relatively long and require intact 5'- and 3'-end sequences to function. We describe a PCR-based protocol for flexible small-scale enzymatic synthesis of such probes. The protocol also offers the advantage over chemical synthesis that longer probes can be made that are densely labeled with detectable functions, resulting in an increased detection signal. The utility of probes synthesized according to this protocol is demonstrated for the analysis of single nucleotide variations in human genomic DNA both in situ and in solution.
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