ArticleNucleic acids research2000
Saccharomyces cerevisiae RAP1 binds to telomeric sequences with spatial flexibility.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Runaway evolution of telomeres in ascomycetous yeasts was accompanied by the replacement of ancestral telomeric proteins.Nucleic acids research · 2025Article
- Subtelomeric elements provide stability to short telomeres in telomerase-negative cells of the budding yeast Naumovozyma castellii.Current genetics · 2025Article
- GenomicLayers: sequence-based simulation of epi-genomes.BMC bioinformatics · 2025Article
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Chromosome-specific telomere lengths and the minimal functional telomere revealed by nanopore sequencing.Genome research · 2022Article
- Article
- Distinct structural groups of histone H3 and H4 residues have divergent effects on chronological lifespan in Saccharomyces cerevisiae.PloS one · 2022Article
- Step-by-Step Evolution of Telomeres: Lessons from Yeasts.Genome biology and evolution · 2021Article
- Telomere shortening triggers a feedback loop to enhance end protection.Nucleic acids research · 2017Article
- A sharp Pif1-dependent threshold separates DNA double-strand breaks from critically short telomeres.eLife · 2017Article
- The wrapping loop and Rap1 C-terminal (RCT) domain of yeast Rap1 modulate access to different DNA binding modes.The Journal of biological chemistry · 2015Article
- Combinatorial recognition of a complex telomere repeat sequence by the Candida parapsilosis Cdc13AB heterodimer.Nucleic acids research · 2015Article
- Telomere DNA recognition in Saccharomycotina yeast: potential lessons for the co-evolution of ssDNA and dsDNA-binding proteins and their target sites.Frontiers in genetics · 2015Review
- Molecular Dynamics Simulation of Rap1 Myb-type domain in Saccharomyces cerevisiae.Bioinformation · 2012Article
- Rap1 binds single-stranded DNA at telomeric double- and single-stranded junctions and competes with Cdc13 protein.The Journal of biological chemistry · 2011Article
- Single- and double-stranded DNA binding proteins act in concert to conserve a telomeric DNA core sequence.Genome integrity · 2011Article
- Rap1 in Candida albicans: an unusual structural organization and a critical function in suppressing telomere recombination.Molecular and cellular biology · 2010Article
- Plasticity of telomere maintenance mechanisms in yeast.Trends in biochemical sciences · 2010Review
- Binding linkage in a telomere DNA-protein complex at the ends of Oxytricha nova chromosomes.Journal of molecular biology · 2005Article
- The different (sur)faces of Rap1p.Molecular genetics and genomics : MGG · 2003Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A wide divergence has been detected in the telomeric sequences among budding yeast species. Despite their length and homogeneity differences, all these yeast telomeric sequences show a conserved core which closely matches the consensus RAP1-binding sequence. We demonstrate that the RAP1 protein binds this sequence core, without involving the diverged sequences outside the core. In Saccharomyces castellii and Saccharomyces dairensis specific classes of interspersed variant repeats are present. We show here that a RAP1-binding site is formed in these species by connecting two consecutive 8 bp telomeric repeats. DNase I footprint analyses specify the binding site as the 13 bp sequence CTGGGTGTCTGGG. The RAP1 protein also binds the variant repeats, although with a lowered affinity. However, a split footprint is produced when RAP1 binds a variant repeat where the two half-sites of the binding site are separated by an additional 6 nt. This is probably caused by the intervening sequence looping out of the RAP1-DNA complex. We suggest that the bipartite subdomain structure of the RAP1 protein allows it to remodel telomeric chromatin, a feature which may be of great relevance for telomeric chromatin assembly and structure in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.