ArticleNucleic acids research2000
Insertional mutagenesis based on illegitimate recombination in Schizosaccharomyces pombe.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 42 citations in OpenAlex.
- Genetic and physical interaction of Ssp1 CaMKK and Rad24 14-3-3 during low pH and osmotic stress in fission yeast.Open biology · 2014Article
- Transposon mutagenesis of the anaerobic commensal, Bacteroides fragilis, using the EZ::TN5 transposome.FEMS microbiology letters · 2012Article
- Generation and analysis of a barcode-tagged insertion mutant library in the fission yeast Schizosaccharomyces pombe.BMC genomics · 2012Article
- The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues.PLoS genetics · 2011Article
- A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe.Nucleic acids research · 2011Article
- H3K9me-independent gene silencing in fission yeast heterochromatin by Clr5 and histone deacetylases.PLoS genetics · 2011Article
- Centaurin-like protein Cnt5 contributes to arsenic and cadmium resistance in fission yeast.FEMS yeast research · 2009Article
- Targeted mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through natural transformation of PCR fragments.Applied and environmental microbiology · 2008Article
- Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in Schizosaccharomyces pombe.Current genetics · 2006Article
- Meiotic chromosome segregation mutants identified by insertional mutagenesis of fission yeast Schizosaccharomyces pombe; tandem-repeat, single-site integrations.Nucleic acids research · 2004Article
- RNA-binding protein Csx1 mediates global control of gene expression in response to oxidative stress.The EMBO journal · 2003Article
- A novel jmjC domain protein modulates heterochromatization in fission yeast.Molecular and cellular biology · 2003Article
- Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient insertional mutagenesis system has been developed for Schizosaccharomyces pombe based on linear PCR-generated cassettes containing selectable markers. It depends upon illegitimate recombination for integration into the genome. Various selectable markers of different sizes can be used to obtain sufficiently high transformation and integration frequencies. Based on Southern blotting, a single insertion is found in each strain and integration sites are broadly distributed in the genome. Sequence analysis of the insert junctions frequently reveals small regions of homology (4-10 bp) between the ends of the integrated cassette and the disrupted gene. The system has been used for simple genetic screens of various types and as a promoter trap for in-frame GFP fusions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.