ArticleProceedings of the National Academy of Sciences of the United States of America2000
A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 106 citations in OpenAlex.
- The N-terminal α helix domain of the mitochondrial VDAC protein Por2 is dispensable for promoting the nuclear localization of yeast AMPK.microPublication biology · 2026Article
- ATP homeostasis and signaling in plants.Plant communications · 2024Review
- Advances inJournal of fungi (Basel, Switzerland) · 2023Review
- Regulatory functions of the Mediator kinases CDK8 and CDK19.Transcription · 2019Review
- Conventional and emerging roles of the energy sensor Snf1/AMPK inMicrobial cell (Graz, Austria) · 2018Review
- Snf1 cooperates with the CWI MAPK pathway to mediate the degradation of Med13 following oxidative stress.Microbial cell (Graz, Austria) · 2018Article
- Ras and Rho GTPase regulation of Pol II transcription: A shortcut model revisited.Transcription · 2017Article
- C-terminal domain (CTD) phosphatase links Rho GTPase signaling to Pol II CTD phosphorylation in Arabidopsis and yeast.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Activation and inhibition of Snf1 kinase activity by phosphorylation within the activation loop.Biochimica et biophysica acta · 2016Article
- Springing into Action: Reg2 Negatively Regulates Snf1 Protein Kinase and Facilitates Recovery from Prolonged Glucose Starvation in Saccharomyces cerevisiae.Applied and environmental microbiology · 2016Article
- Glucose repression in Saccharomyces cerevisiae.FEMS yeast research · 2015Review
- The yeast AMPK homolog SNF1 regulates acetyl coenzyme A homeostasis and histone acetylation.Molecular and cellular biology · 2013Article
- Ectopic expression of a novel Ser/Thr protein kinase from cotton (Gossypium barbadense), enhances resistance to Verticillium dahliae infection and oxidative stress in Arabidopsis.Plant cell reports · 2013Article
- Cyclin-dependent kinase E1 (CDKE1) provides a cellular switch in plants between growth and stress responses.The Journal of biological chemistry · 2013Article
- Mitochondrial porin Por1 and its homolog Por2 contribute to the positive control of Snf1 protein kinase in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- The AMP-activated protein kinase Snf1 regulates transcription factor binding, RNA polymerase II activity, and mRNA stability of glucose-repressed genes in Saccharomyces cerevisiae.The Journal of biological chemistry · 2012Article
- Integrated analysis of transcriptome and lipid profiling reveals the co-influences of inositol-choline and Snf1 in controlling lipid biosynthesis in yeast.Molecular genetics and genomics : MGG · 2012Article
- Snf1/AMPK regulates Gcn5 occupancy, H3 acetylation and chromatin remodelling at S. cerevisiae ADY2 promoter.Biochimica et biophysica acta · 2012Article
- Protein kinase A contributes to the negative control of Snf1 protein kinase in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- Mediator acts upstream of the transcriptional activator Gal4.PLoS biology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
RNA polymerase II holoenzymes respond to activators and repressors that are regulated by signaling pathways. Here we present evidence for a "shortcut" mechanism in which the Snf1 protein kinase of the glucose signaling pathway directly regulates transcription by the yeast holoenzyme. In response to glucose limitation, the Snf1 kinase stimulates transcription by holoenzyme that has been artificially recruited to a reporter by a LexA fusion to a holoenzyme component. We show that Snf1 interacts physically with the Srb/mediator proteins of the holoenzyme in both two-hybrid and coimmunoprecipitation assays. We also show that a catalytically hyperactive Snf1, when bound to a promoter as a LexA fusion protein, activates transcription in a glucose-regulated manner; moreover, this activation depends on the integrity of the Srb/mediator complex. These results suggest that direct regulatory interactions between signal transduction pathways and RNA polymerase II holoenzyme provide a mechanism for transcriptional control in response to important signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.