ArticleProceedings of the National Academy of Sciences of the United States of America2000
Mechanisms that direct ordered assembly of T cell receptor beta locus V, D, and J gene segments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
- Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Helix-Loop-Helix Proteins in Adaptive Immune Development.Frontiers in immunology · 2022Review
- Poor quality Vβ recombination signal sequences stochastically enforce TCRβ allelic exclusion.The Journal of experimental medicine · 2020Article
- Replacement of TCR Dβ With Immunoglobulin DFrontiers in immunology · 2020Article
- Preimmune Control of the Variance of TCR CDR-B3: Insights Gained From Germline Replacement of a TCR Dβ Gene Segment With an Ig DFrontiers in immunology · 2020Article
- A Lamina-Associated Domain Border Governs Nuclear Lamina Interactions, Transcription, and Recombination of the Tcrb Locus.Cell reports · 2018Article
- Analyzing the CDR3 Repertoire with respect to TCR-Beta Chain V-D-J and V-J Rearrangements in Peripheral T Cells using HTS.Scientific reports · 2016Article
- Specification of Vδ and Vα usage by Tcra/Tcrd locus V gene segment promoters.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Synapsis alters RAG-mediated nicking at Tcrb recombination signal sequences: implications for the “beyond 12/23” rule.Molecular and cellular biology · 2014Article
- The center of accessibility: Dβ control of V(D)J recombination.Archivum immunologiae et therapiae experimentalis · 2010Review
- Assembled DJ beta complexes influence TCR beta chain selection and peripheral V beta repertoire.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Article
- Mechanics of T cell receptor gene rearrangement.Current opinion in immunology · 2009Review
- Article
- Functional overlap in the cis-acting regulation of the V(D)J recombination at the TCRbeta locus.Molecular immunology · 2009Article
- Productive coupling of accessible Vbeta14 segments and DJbeta complexes determines the frequency of Vbeta14 rearrangement.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- The beyond 12/23 restriction is imposed at the nicking and pairing steps of DNA cleavage during V(D)J recombination.Molecular and cellular biology · 2007Article
- Increased frequency of aberrant V(D)J recombination products in core RAG-expressing mice.Nucleic acids research · 2004Article
- Impaired V(D)J recombination and lymphocyte development in core RAG1-expressing mice.The Journal of experimental medicine · 2003Article
- T cell receptor (TCR) alpha/delta locus enhancer identity and position are critical for the assembly of TCR delta and alpha variable region genes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
T cell receptor (TCR) beta variable region genes are assembled in progenitor T cells from germ-line Vbeta, Dbeta, and Jbeta segments via an ordered two-step process in which Dbeta to Jbeta rearrangements occur on both alleles before appendage of a Vbeta to a preexisting DJbeta complex. Direct joining of Vbeta segments to nonrearranged Dbeta or Jbeta segments, while compatible with known restrictions on the V(D)J recombination mechanism, are infrequent within the endogenous TCRbeta locus. We have analyzed mechanisms that mediate ordered Vbeta, Dbeta, and Jbeta assembly via an approach in which TCRbeta minilocus recombination substrates were introduced into embryonic stem cells and then analyzed for rearrangement in normal thymocytes by recombinase-activating gene 2-deficient blastocyst complementation. These analyses demonstrated that Vbeta segments are preferentially targeted for rearrangement to Dbeta as opposed to Jbeta segments. In addition, we further demonstrated that Vbeta segments can be appended to nonrearranged endogenous Dbeta segments in which we have eliminated the ability of Dbeta segments to join to Jbeta segments. Our findings are discussed in the context of the mechanisms that regulate the ordered assembly and utilization of V, D, and J segments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.