ArticleJournal of bacteriology2000
The flagellar hook protein, FlgE, of Salmonella enterica serovar typhimurium is posttranscriptionally regulated in response to the stage of flagellar assembly.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Long-read methylome analysis of Oleidesulfovibrio alaskensis G20 biofilm under copper stress.Scientific reports · 2025Article
- Modulation of the Enzymatic Activity of the Flagellar Lytic Transglycosylase SltF by Rod Components and the Scaffolding Protein FlgJ inJournal of bacteriology · 2021Article
- Flagellar Motility Is Critical forFrontiers in microbiology · 2020Article
- Characterization of FlgP, an Essential Protein for Flagellar Assembly inJournal of bacteriology · 2019Article
- Molecular Prediction of the O157:H-Negative Phenotype Prevalent in Australian Shiga Toxin-Producing Escherichia coli Cases Improves Concordance ofJournal of clinical microbiology · 2018Article
- First insights into the pleiotropic role of vrf (yedF), a newly characterized gene of Salmonella Typhimurium.Scientific reports · 2017Article
- Biochemical Characterization of the Flagellar Rod Components of Rhodobacter sphaeroides: Properties and Interactions.Journal of bacteriology · 2016Article
- Initial characterization of the FlgE hook high molecular weight complex of Borrelia burgdorferi.PloS one · 2014Article
- Molecular characterization of the flagellar hook in Bacillus subtilis.Journal of bacteriology · 2012Article
- A single-domain FlgJ contributes to flagellar hook and filament formation in the Lyme disease spirochete Borrelia burgdorferi.Journal of bacteriology · 2012Article
- CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.Molecular microbiology · 2011Article
- Posttranscriptional control of the Salmonella enterica flagellar hook protein FlgE.Journal of bacteriology · 2006Article
- The decrease in FlaA observed in a flaB mutant of Borrelia burgdorferi occurs posttranscriptionally.Journal of bacteriology · 2004Article
- Isolation and expression analysis of the testis-specific gene, human OPPO1.Journal of assisted reproduction and genetics · 2004Article
- Porphyromonas gingivalis genes involved in fimA regulation.Infection and immunity · 2004Article
- Type III secretion systems and bacterial flagella: insights into their function from structural similarities.Proceedings of the National Academy of Sciences of the United States of America · 2003Review
- Asymmetrical flagellar rotation in Borrelia burgdorferi nonchemotactic mutants.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Coupling of flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2000Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We investigated the posttranscriptional regulation of flgE, a class 2 gene that encodes the hook subunit protein of the flagella. RNase protection assays demonstrated that the flgE gene was transcribed at comparable levels in numerous strains defective in known steps of flagellar assembly. However, Western analyses of these strains demonstrated substantial differences in FlgE protein levels. Although wild-type FlgE levels were observed in strains with deletions of genes encoding components of the switch complex and the flagellum-specific secretion apparatus, no protein was detected in a strain with deletions of the rod, ring, and hook-associated proteins. To determine whether FlgE levels were affected by the stage of hook-basal-body assembly, Western analysis was performed on strains with mutations at individual loci encompassed by the deletion. FlgE protein was undetectable in rod mutants, intermediate in ring mutants, and wild type in hook-associated protein mutants. The lack of negative regulation in switch complex and flagellum-specific secretion apparatus deletion mutants blocked for flagellar construction prior to rod assembly suggests that these structures play a role in the negative regulation of FlgE. Quantitative Western analyses of numerous flagellar mutants indicate that FlgE levels reflect the stage at which flagellar assembly is blocked. These data provide evidence for negative posttranscriptional regulation of FlgE in response to the stage of flagellar assembly.
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