ArticleJournal of bacteriology2000
A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 78 citations in OpenAlex.
- Three alginate lyases provide a new gutApplied and environmental microbiology · 2023Article
- Substrate size-dependent conformational changes of bacterial pectin-binding protein crucial for chemotaxis and assimilation.Scientific reports · 2022Article
- Sphingopyxis lutea sp. nov., a novel moderately halotolerant bacterium isolated from pebbles.Archives of microbiology · 2022Article
- 4-Deoxy-l-Molecules (Basel, Switzerland) · 2022Review
- Bacteria with a mouth: Discovery and new insights into cell surface structure and macromolecule transport.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Article
- Bacterial alginate metabolism: an important pathway for bioconversion of brown algae.Biotechnology for biofuels · 2021Review
- Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in amBio · 2020Article
- A solute-binding protein in the closed conformation induces ATP hydrolysis in a bacterial ATP-binding cassette transporter involved in the import of alginate.The Journal of biological chemistry · 2017Article
- Biofuel Production Based on Carbohydrates from Both Brown and Red Macroalgae: Recent Developments in Key Biotechnologies.International journal of molecular sciences · 2016Review
- Site-Directed Mutagenesis-Based Functional Analysis and Characterization of Endolytic Lyase Activity of N- and C-Terminal Domains of a Novel Oligoalginate Lyase from Sphingomonas sp. MJ-3 Possessing Exolytic Lyase Activity in the Intact Enzyme.Marine biotechnology (New York, N.Y.) · 2015Article
- Genome Sequence of Sphingobium yanoikuyae B1, a Polycyclic Aromatic Hydrocarbon-Degrading Strain.Genome announcements · 2015Article
- Structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism.The Journal of biological chemistry · 2014Article
- Alginate-dependent gene expression mechanism in Sphingomonas sp. strain A1.Journal of bacteriology · 2014Article
- DNA regions responsible for maintenance of Shingobium plasmid pYAN-2.Microbes and environments · 2014Article
- Comparative characterization of two marine alginate lyases from Zobellia galactanivorans reveals distinct modes of action and exquisite adaptation to their natural substrate.The Journal of biological chemistry · 2013Article
- Difference in Degradation Patterns on Inulin-type Fructans among Strains of Lactobacillus delbrueckii and Lactobacillus paracasei.Bioscience of microbiota, food and health · 2013Article
- Genome sequence of Sphingomonas wittichii DP58, the first reported phenazine-1-carboxylic acid-degrading strain.Journal of bacteriology · 2012Article
- Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein Algp7 isolated from Sphingomonas sp. A1.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- Origin and diversity of alginate lyases of families PL-5 and -7 in Sphingomonas sp. strain A1.Journal of bacteriology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A gram-negative bacterium, Sphingomonas sp. strain A1, isolated as a producer of alginate lyase, has a characteristic cell envelope structure and forms a mouth-like pit on its surface. The pit is produced only when the cells have to incorporate and assimilate alginate. An alginate uptake-deficient mutant was derived from cells of strain A1. One open reading frame, algS (1,089 bp), exhibiting homology to the bacterial ATP-binding domain of an ABC transporter, was cloned as a fragment complementing the mutation. algS was followed by two open reading frames, algM1 (972 bp) and algM2 (879 bp), which exhibit homology with the transmembrane permeases of ABC transporters. Disruption of algS of strain A1 resulted in the failure to incorporate alginate and to form a pit. Hexahistidine-tagged AlgS protein (AlgS(His6)) overexpressed in Escherichia coli and purified by Ni(2+) affinity column chromatography showed ATPase activity. Based on these results, we propose the occurrence of a novel pit-dependent ABC transporter system that allows the uptake of macromolecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.