ArticleDiabetes care2000
Evaluation of HbA1c determination methods in patients with hemoglobinopathies.
Article in Diabetes care, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 122 citations in OpenAlex.
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- A case of α-chain variant hemoglobin (Hb Chad) with falsely high HbA1c levels measured by immunoassay.Diabetology international · 2022Article
- Inverse Association between Iron Deficiency and Glycated Hemoglobin Levels in Ghanaian Adults-the RODAM Study.The Journal of nutrition · 2020Article
- A case of variant hemoglobin (Hb Agenogi) with type 2 diabetes mellitus showed high HbA1c levels measured by immunoassay due to enhanced antigenicity.Diabetology international · 2019Article
- Glycosylated haemoglobin: a false sense of security.BMJ case reports · 2018Article
- Hemoglobin Providence (β82 Lys > Asn, Asp) and lower-than-expected HbA1c in a nonadherent teenager with type 1 diabetes: a case report and literature review.Clinical case reports · 2017Article
- Efficacy, safety and influencing factors of intra-calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus-induced lower extremity vascular disease.Experimental and therapeutic medicine · 2017Article
- Current Status of HbA1c Biosensors.Sensors (Basel, Switzerland) · 2017Review
- Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies.Scientifica · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate commercially available determination methods for HbA1c in patients with hemoglobin variants. RESEARCH DESIGN AND
methodsHbA1c values were determined with various commercially available methods, including ion-exchange high-performance liquid chromatography (HPLC), boronate affinity assay, and immunoagglutination in patients with the hemoglobin mutations Hb Graz, Hb Sherwood Forest, Hb O Padova, Hb D, and Hb S.
resultsThe effect of hemoglobinopathies on glycohemoglobin measurements was highly method dependent. The HPLC methods for HbA1c determination lacked the resolution necessary to differentiate hemoglobin variants. They demonstrated additional peaks in the chromatograms and HbA1c results either too low or too high compared with the nondiabetic reference range. With all immunoassays, Hb Graz demonstrated falsely low values. The other hemoglobinopathies in our study caused falsely low and/or high HbA1c results in immunoagglutination methods. The boronate affinity method showed values in an acceptable range for all hemoglobin variants.
conclusionsBecause of the local occurrence of Hb variants and the ethnic origin of a given population, every individual laboratory must establish and validate its own assay method. In managing diabetic patients, knowledge of hemoglobinopathies influencing HbA1c determination methods is essential because hemoglobin variants could cause mismanagement of diabetes resulting from false HbA1c determinations.
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