ArticleBiophysical journal2000
Translocation-independent dimerization of the EcoKI endonuclease visualized by atomic force microscopy.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- AFM Investigation of the Influence of Steam Flow through a Conical Coil Heat Exchanger on Enzyme Properties.Micromachines · 2022Article
- Single molecular investigation of DNA looping and aggregation by restriction endonuclease BspMI.Scientific reports · 2014Article
- An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme.Nucleic acids research · 2011Article
- The single polypeptide restriction-modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops.Nucleic acids research · 2009Article
- The fragment structure of a putative HsdR subunit of a type I restriction enzyme from Vibrio vulnificus YJ016: implications for DNA restriction and translocation activity.Nucleic acids research · 2009Article
- Type I restriction endonucleases are true catalytic enzymes.Nucleic acids research · 2009Article
- Atomic force microscopy of the EcoKI Type I DNA restriction enzyme bound to DNA shows enzyme dimerization and DNA looping.Nucleic acids research · 2009Article
- The structure of M.EcoKI Type I DNA methyltransferase with a DNA mimic antirestriction protein.Nucleic acids research · 2009Article
- EcoR124I: from plasmid-encoded restriction-modification system to nanodevice.Microbiology and molecular biology reviews : MMBR · 2008Review
- DNA looping and translocation provide an optimal cleavage mechanism for the type III restriction enzymes.The EMBO journal · 2007Article
- Nuclear envelope: nanoarray responsive to aldosterone.The Journal of membrane biology · 2004Review
- Alleviation of restriction by DNA condensation and non-specific DNA binding ligands.Nucleic acids research · 2004Article
- DNA communications by Type III restriction endonucleases--confirmation of 1D translocation over 3D looping.Nucleic acids research · 2004Article
- Tracking EcoKI and DNA fifty years on: a golden story full of surprises.Nucleic acids research · 2003Article
- Structural perturbations in DNA caused by bis-intercalation of ditercalinium visualised by atomic force microscopy.Nucleic acids research · 2002Article
- Aldosterone signaling pathway across the nuclear envelope.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Direct visualization of ligand-protein interactions using atomic force microscopy.British journal of pharmacology · 2002Article
- Nucleoside triphosphate-dependent restriction enzymes.Nucleic acids research · 2001Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial type I restriction/modification systems are capable of performing multiple actions in response to the methylation pattern on their DNA recognition sequences. The enzymes making up these systems serve to protect the bacterial cells against viral infection by binding to their recognition sequences on the invading DNA and degrading it after extensive ATP-driven translocation. DNA cleavage has been thought to occur as the result of a collision between two translocating enzyme complexes. Using atomic force microscopy (AFM), we show here that EcoKI dimerizes rapidly when bound to a plasmid containing two recognition sites for the enzyme. Dimerization proceeds in the absence of ATP and is also seen with an EcoKI mutant (K477R) that is unable to translocate DNA. Only monomers are seen when the enzyme complex binds to a plasmid containing a single recognition site. Based on our results, we propose that the binding of EcoKI to specific DNA target sequences is accompanied by a conformational change that leads rapidly to dimerization. This event is followed by ATP-dependent translocation and cleavage of the DNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.