ArticleBiophysical journal2000
Proton-sensitive transitions of renal type II Na(+)-coupled phosphate cotransporter kinetics.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Structures of the sodium-coupled phosphate importer SLC34A2 reveal a distinct architecture and gating mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Topology, tissue distribution, and transcriptional level of SLC34s in response to Pi and pH in grass carp Ctenopharyngodon idella.Fish physiology and biochemistry · 2021Article
- Mechanisms of phosphate transport.Nature reviews. Nephrology · 2019Review
- Physiological regulation of phosphate by vitamin D, parathyroid hormone (PTH) and phosphate (Pi).Pflugers Archiv : European journal of physiology · 2019Review
- The molecular mechanism of SLC34 proteins: insights from two decades of transport assays and structure-function studies.Pflugers Archiv : European journal of physiology · 2019Review
- DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.Physiological reviews · 2017Review
- Effect of Potassium Citrate on Calcium Phosphate Stones in a Model of Hypercalciuria.Journal of the American Society of Nephrology : JASN · 2015Article
- The SLC34 family of sodium-dependent phosphate transporters.Pflugers Archiv : European journal of physiology · 2014Review
- Conferring electrogenicity to the electroneutral phosphate cotransporter NaPi-IIc (SLC34A3) reveals an internal cation release step.Pflugers Archiv : European journal of physiology · 2013Article
- Substrate interactions of the electroneutral Na+-coupled inorganic phosphate cotransporter (NaPi-IIc).The Journal of physiology · 2009Article
- Temperature dependence of steady-state and presteady-state kinetics of a type IIb Na+/P i cotransporter.The Journal of membrane biology · 2007Article
- Voltage clamp fluorometric measurements on a type II Na+-coupled Pi cotransporter: shedding light on substrate binding order.The Journal of general physiology · 2006Article
- Electrogenic kinetics of a mammalian intestinal type IIb Na(+)/P(i) cotransporter.The Journal of membrane biology · 2006Article
- Renouncing electroneutrality is not free of charge: switching on electrogenicity in a Na+-coupled phosphate cotransporter.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Structure-function relations of the first and fourth extracellular linkers of the type IIa Na+/Pi cotransporter: II. Substrate interaction and voltage dependency of two functionally important sites.The Journal of general physiology · 2004Article
- Essential cysteine residues of the type IIa Na+/Pi cotransporter.Pflugers Archiv : European journal of physiology · 2003Article
- The relation between charge movement and transport-associated currents in the rat GABA cotransporter rGAT1.The Journal of physiology · 2002Article
- Role of anion-cation interactions on the pre-steady-state currents of the rat Na(+)-Cl(-)-dependent GABA cotransporter rGAT1.The Journal of physiology · 2002Article
- Cysteine mutagenesis reveals novel structure-function features within the predicted third extracellular loop of the type IIa Na(+)/P(i) cotransporter.The Journal of general physiology · 2001Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the kidney proximal tubule, acidification of the glomerular filtrate leads to an inhibition of inorganic phosphate (P(i)) reabsorption by type II Na(+)-coupled cotransporters (NaPi-II). As external pH also alters the divalent/monovalent P(i) ratio, it has been difficult to separate putative proton interactions with the cotransporter from direct titration of divalent P(i), the preferred species transported. To distinguish between these possibilities and identify pH-sensitive transitions in the cotransport cycle, the pH-dependent kinetics of two NaPi-II isoforms, expressed in Xenopus laevis oocytes, were investigated electrophysiologically. At -50 mV, both isoforms showed >70% suppression of electrogenic response for an external pH change from 8.0 to 6.2, not attributable to titration of divalent P(i). This was accompanied by a progressive removal of steady-state voltage dependence. The NaPi-II-related uncoupled slippage current was unaffected by a pH change from 7.4 to 6.2, with no shift in the reversal potential, which suggested that protons do not function as substrate. The voltage-dependence of pre-steady-state relaxations was shifted to depolarizing potentials in 100 mM and 0 mM Na(ext)(+) and two kinetic components were resolved, the slower of which was pH-dependent. The changes in kinetics are predicted by a model in which protons interact with the empty carrier and final Na(+) binding step.
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