ArticleBiophysical journal2000
An image-based model of calcium waves in differentiated neuroblastoma cells.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 139 citations in OpenAlex.
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- How Merkel cells transduce mechanical stimuli: A biophysical model of Merkel cells.PLoS computational biology · 2023Article
- Impact of Interdependent CaCell biochemistry and biophysics · 2023Article
- Beyond analytic solution: Analysis of FRAP experiments by spatial simulation of the forward problem.Biophysical journal · 2023Article
- Mechanistic insights of neuronal calcium and IPEuropean biophysics journal : EBJ · 2023Article
- Heterogeneous off-target impact of ion-channel deletion on intrinsic properties of hippocampal model neurons that self-regulate calcium.Frontiers in cellular neuroscience · 2023Article
- Calcium modeling of spine apparatus-containing human dendritic spines demonstrates an "all-or-nothing" communication switch between the spine head and dendrite.PLoS computational biology · 2022Article
- Ten steps to investigate a cellular system with mathematical modeling.PLoS computational biology · 2021Article
- CaeLife · 2020Article
- Three dimensional coupled reaction-diffusion modeling of calcium and inositol 1,4,5-trisphosphate dynamics in cardiomyocytes.RSC advances · 2019Article
- Intracellular calcium stores mediate metaplasticity at hippocampal dendritic spines.The Journal of physiology · 2019Article
- Active dendrites regulate the spatiotemporal spread of signaling microdomains.PLoS computational biology · 2018Article
- Spine-to-Dendrite Calcium Modeling Discloses Relevance for Precise Positioning of Ryanodine Receptor-Containing Spine Endoplasmic Reticulum.Scientific reports · 2018Article
- Disentangling astroglial physiology with a realistic cell model in silico.Nature communications · 2018Article
- A biophysically detailed computational model of urinary bladder small DRG neuron soma.PLoS computational biology · 2018Article
- TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1.The Journal of cell biology · 2017Article
- Degeneracy in the regulation of short-term plasticity and synaptic filtering by presynaptic mechanisms.The Journal of physiology · 2017Article
- The Effect of Gap Junctional Coupling on the Spatiotemporal Patterns of Ca2+ Signals and the Harmonization of Ca2+-Related Cellular Responses.PLoS computational biology · 2016Article
- Routes of Ca2+ Shuttling during Ca2+ Oscillations: FOCUS ON THE ROLE OF MITOCHONDRIAL Ca2+ HANDLING AND CYTOSOLIC Ca2+ BUFFERS.The Journal of biological chemistry · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Calcium waves produced by bradykinin-induced inositol-1,4, 5-trisphosphate (InsP(3))-mediated release from endoplasmic reticulum (ER) have been imaged in N1E-115 neuroblastoma cells. A model of this process was built using the "virtual cell," a general computational system for integrating experimental image, biochemical, and electrophysiological data. The model geometry was based on a cell for which the calcium wave had been experimentally recorded. The distributions of the relevant cellular components [InsP(3) receptor (InsP(3)R)], sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) pumps, bradykinin receptors, and ER] were based on 3D confocal immunofluorescence images. Wherever possible, known biochemical and electrophysiological data were used to constrain the model. The simulation closely matched the spatial and temporal characteristics of the experimental calcium wave. Predictions on different patterns of calcium signals after InsP(3) uncaging or for different cell geometries were confirmed experimentally, thus helping to validate the model. Models in which the spatial distributions of key components are altered suggest that initiation of the wave in the center of the neurite derives from an interplay of soma-biased ER distribution and InsP(3) generation biased toward the neurite. Simulations demonstrate that mobile buffers (like the indicator fura-2) significantly delay initiation and lower the amplitude of the wave. Analysis of the role played by calcium diffusion indicated that the speed of the wave is only slightly dependent on the ability of calcium to diffuse to and activate neighboring InsP(3) receptor sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.