ArticleBiophysical journal2000
Mitochondrial calcium transients in adult rabbit cardiac myocytes: inhibition by ruthenium red and artifacts caused by lysosomal loading of Ca(2+)-indicating fluorophores.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 140 citations in OpenAlex.
- Mechanism of action behind the pain-relief effects of extracellular vesicles in microfragmented adipose tissue: an in vitro and in vivo study.Journal of translational medicine · 2025Article
- Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2024Review
- Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart.Cell reports · 2021Article
- mTOR-mediated calcium transients affect cardiac function in ex vivo ischemia-reperfusion injury.Physiological reports · 2021Article
- Pharmacological inhibition of the mitochondrial CaJournal of molecular and cellular cardiology · 2021Review
- Three-Week-Old Rabbit Ventricular Cardiomyocytes as a Novel System to Study Cardiac Excitation and EC Coupling.Frontiers in physiology · 2021Article
- Mitochondrial Calcium Uniporter Deficiency in Zebrafish Causes Cardiomyopathy With Arrhythmia.Frontiers in physiology · 2020Article
- The Role of Mitochondria in the Mechanisms of Cardiac Ischemia-Reperfusion Injury.Antioxidants (Basel, Switzerland) · 2019Review
- Mitochondrial CaFEBS letters · 2019Review
- SR-mitochondria communication in adult cardiomyocytes: A close relationship where the CaArchives of biochemistry and biophysics · 2019Review
- Intramitochondrial Ascorbic Acid Enhances the Formation of Mitochondrial Superoxide Induced by Peroxynitrite via a CaInternational journal of molecular sciences · 2017Article
- Tissue-Specific Mitochondrial Decoding of Cytoplasmic CaCell reports · 2017Article
- A technical review of optical mapping of intracellular calcium within myocardial tissue.American journal of physiology. Heart and circulatory physiology · 2016Review
- SERCA2a upregulation ameliorates cellular alternans induced by metabolic inhibition.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Article
- Hydrogen peroxide-induced calcium influx in lung microvascular endothelial cells involves TRPV4.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Inhibition of NAADP signalling on reperfusion protects the heart by preventing lethal calcium oscillations via two-pore channel 1 and opening of the mitochondrial permeability transition pore.Cardiovascular research · 2015Article
- Mangiferin induces cell death against rhabdomyosarcoma through sustained oxidative stress.Integrative medicine research · 2015Article
- Calcium movement in cardiac mitochondria.Biophysical journal · 2014Article
- NCLX: the mitochondrial sodium calcium exchanger.Journal of molecular and cellular cardiology · 2013Article
- Calcium signaling in cardiac mitochondria.Journal of molecular and cellular cardiology · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A cold/warm loading protocol was used to ester-load Rhod 2 into mitochondria and other organelles and Fluo 3 into the cytosol of adult rabbit cardiac myocytes for confocal fluorescence imaging. Transient increases in both cytosolic Fluo 3 and mitochondrial Rhod 2 fluorescence occurred after electrical stimulation. Ruthenium red, a blocker of the mitochondrial Ca(2+) uniporter, inhibited mitochondrial Rhod 2 fluorescence transients but not cytosolic Fluo 3 transients. Thus the ruthenium red-sensitive mitochondrial Ca(2+) uniporter catalyzes Ca(2+) uptake during beat-to-beat transients of mitochondrial free Ca(2+), which in turn may help match mitochondrial ATP production to myocardial ATP demand. After ester loading, substantial amounts of Ca(2+)-indicating fluorophores localized into an acidic lysosomal/endosomal compartment. This lysosomal fluorescence did not respond to electrical stimulation. Because fluorescence arose predominantly from lysosomes after the cold loading/warm incubation procedure, total cellular fluorescence failed to track beat-to-beat changes of mitochondrial fluorescence. Only three-dimensionally resolved confocal imaging distinguished the relatively weak mitochondrial signal from the bright lysosomal fluorescence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.