ArticleMolecular and cellular biology2000
Akr1p and the type I casein kinases act prior to the ubiquitination step of yeast endocytosis: Akr1p is required for kinase localization to the plasma membrane.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 113 citations in OpenAlex.
- Genome-wide identification, structural analysis, and heterologous functional inference of FvPAT10, a protein S-acyl transferase in woodland strawberry (Fragaria vesca).BMC plant biology · 2025Article
- Casein kinase 1 controls components of a TORC2 signaling network in budding yeast.Journal of cell science · 2024Article
- Casein kinase 1 controls components of a TORC2 signaling network in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Palmitoyl Transferase FonPAT2-Catalyzed Palmitoylation of the FonAP-2 Complex Is Essential for Growth, Development, Stress Response, and Virulence in Fusarium oxysporum f. sp.Microbiology spectrum · 2023Article
- Overcoming Fungal Echinocandin Resistance through Inhibition of the Non-essential Stress Kinase Yck2.Cell chemical biology · 2020Article
- The casein kinase MoYck1 regulates development, autophagy, and virulence in the rice blast fungus.Virulence · 2019Article
- Protein Palmitoylation Plays an Important Role inMolecular & cellular proteomics : MCP · 2018Article
- Cysteine desulfurase is regulated by phosphorylation of Nfs1 in yeast mitochondria.Mitochondrion · 2018Article
- Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes.Memorias do Instituto Oswaldo Cruz · 2018Article
- Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters.FEMS microbiology letters · 2017Article
- Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae.Applied and environmental microbiology · 2017Article
- Protein S-Acyltransferase 14: A Specific Role for Palmitoylation in Leaf Senescence in Arabidopsis.Plant physiology · 2016Article
- Cu/Zn superoxide dismutase and the proton ATPase Pma1p of Saccharomyces cerevisiae.Biochemical and biophysical research communications · 2015Article
- Specific α-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2.Molecular and cellular biology · 2014Article
- Mitochondrial protein import under kinase surveillance.Microbial cell (Graz, Austria) · 2014Review
- Genome-wide identification and comprehensive analyses of the kinomes in four pathogenic microsporidia species.PloS one · 2014Article
- The CK1 Family: Contribution to Cellular Stress Response and Its Role in Carcinogenesis.Frontiers in oncology · 2014Review
- The glucose signaling network in yeast.Biochimica et biophysica acta · 2013Review
- A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis.The New phytologist · 2013Article
- Pkh1/2-dependent phosphorylation of Vps27 regulates ESCRT-I recruitment to endosomes.Molecular biology of the cell · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ubiquitination of the plasma membrane-localized yeast a-factor receptor (Ste3p) triggers a rapid, ligand-independent endocytosis leading to its vacuolar degradation. This report identifies two mutants that block uptake by blocking ubiquitination, these being mutant either for the ankyrin repeat protein Akr1p or for the redundant type I casein kinases Yck1p and Yck2p. While no obvious defect was seen for wild-type Ste3p phosphorylation in akr1 or yck mutant backgrounds, examination of the Delta320-413 Ste3p deletion mutant phosphorylation did reveal a clear defect in both mutants. The Delta320-413 deletion removes 18 Ser-Thr residues (possible YCK-independent phosphorylation sites) yet retains the 15 Ser-Thr residues of the Ste3p PEST-like ubiquitination-endocytosis signal. Two other phenotypes link akr1 and yck mutants: both are defective in phosphorylation of wild-type alpha-factor receptor, and while both are defective for Ste3p constitutive internalization, both remain partially competent for the Ste3p ligand-dependent uptake mode. Yck1p-Yck2p may be the function responsible in phosphorylation of the PEST-like ubiquitination-endocytosis signal. Akr1p appears to function in localizing Yck1p-Yck2p to the plasma membrane, a localization that depends on prenylation of C-terminal dicysteinyl motifs. In akr1Delta cells, Yck2p is mislocalized, showing a diffuse cytoplasmic localization identical to that seen for a Yck2p mutant that lacks the C-terminal Cys-Cys, indicating a likely Akr1p requirement for the lipid modification of Yck2p, for prenylation, or possibly for palmitoylation.
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