ArticleMolecular and cellular biology2000
A pentamer transcriptional complex including tal-1 and retinoblastoma protein downmodulates c-kit expression in normal erythroblasts.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Integrative proteomics reveals principles of dynamic phosphosignaling networks in human erythropoiesis.Molecular systems biology · 2020Article
- The histone H3K4 demethylase JARID1A directly interacts with haematopoietic transcription factor GATA1 in erythroid cells through its second PHD domain.Royal Society open science · 2020Article
- Article
- LMO2 at 25 years: a paradigm of chromosomal translocation proteins.Open biology · 2015Review
- Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors.Cell death and differentiation · 2014Article
- Solution structure of a tethered Lmo2(LIM2) /Ldb1(LID) complex.Protein science : a publication of the Protein Society · 2012Article
- Targeting LMO2 with a peptide aptamer establishes a necessary function in overt T-cell neoplasia.Cancer research · 2009Article
- Erythropoietin down-regulates stem cell factor receptor (Kit) expression in the leukemic proerythroblast: role of Lyn kinase.PloS one · 2009Article
- MicroRNA 223-dependent expression of LMO2 regulates normal erythropoiesis.Haematologica · 2009Article
- Discovering sequence motifs with arbitrary insertions and deletions.PLoS computational biology · 2008Article
- A positive role for NLI/Ldb1 in long-range beta-globin locus control region function.Molecular cell · 2007Article
- Article
- TAL-1/SCL and its partners E47 and LMO2 up-regulate VE-cadherin expression in endothelial cells.Molecular and cellular biology · 2007Article
- MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- ETO-2 associates with SCL in erythroid cells and megakaryocytes and provides repressor functions in erythropoiesis.Molecular and cellular biology · 2005Article
- Functional but abnormal adult erythropoiesis in the absence of the stem cell leukemia gene.Molecular and cellular biology · 2005Article
- Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex.Nucleic acids research · 2005Article
- Decoding hematopoietic specificity in the helix-loop-helix domain of the transcription factor SCL/Tal-1.Molecular and cellular biology · 2004Article
- Cell-nonautonomous function of the retinoblastoma tumour suppressor protein: new interpretations of old phenotypes.EMBO reports · 2002Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human proerythroblasts and early erythroblasts, generated in vitro by normal adult progenitors, contain a pentamer protein complex comprising the tal-1 transcription factor heterodimerized with the ubiquitous E2A protein and linked to Lmo2, Ldb1, and retinoblastoma protein (pRb). The pentamer can assemble on a consensus tal-1 binding site. In the pRb(-) SAOS-2 cell line transiently transfected with a reporter plasmid containing six tal-1 binding site, pRb enhances the transcriptional activity of tal-1-E12-Lmo2 and tal-1-E12-Lmo2-Ldb1 complexes but not that of a tal-1-E12 heterodimer. We explored the functional significance of the pentamer in erythropoiesis, specifically, its transcriptional effect on the c-kit receptor, a tal-1 target gene stimulating early hematopoietic proliferation downmodulated in erythroblasts. In TF1 cells, the pentamer decreased the activity of the reporter plasmid containing the c-kit proximal promoter with two inverted E box-2 type motifs. In SAOS-2 cells the pentamer negatively regulates (i) the activity of the reporter plasmid containing the proximal human c-kit promoter and (ii) endogenous c-kit expression. In both cases pRb significantly potentiates the inhibitory effect of the tal-1-E12-Lmo2-Ldb1 tetramer. These data indicate that this pentameric complex assembled in maturing erythroblasts plays an important regulatory role in c-kit downmodulation; hypothetically, the complex may regulate the expression of other critical erythroid genes.
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