ArticleMolecular and cellular biology2000
The C terminus of the Saccharomyces cerevisiae alpha-factor receptor contributes to the formation of preactivation complexes with its cognate G protein.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Cell-internal autocrine receptor inactivation supports maintenance of mating-type identity in yeast.Science advances · 2026Article
- Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast α-Factor Receptor.Biomolecules · 2022Review
- A Paradigm for Peptide Hormone-GPCR Analyses.Molecules (Basel, Switzerland) · 2020Review
- Yeast GPCR signaling reflects the fraction of occupied receptors, not the number.Molecular systems biology · 2016Article
- Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.The Journal of biological chemistry · 2016Article
- Cell penetrating peptides and cationic antibacterial peptides: two sides of the same coin.The Journal of biological chemistry · 2014Article
- Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis.Biology open · 2014Article
- Functional coupling of a nematode chemoreceptor to the yeast pheromone response pathway.PloS one · 2014Article
- Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast.Protein engineering, design & selection : PEDS · 2013Article
- Functional and physical interactions among Saccharomyces cerevisiae α-factor receptors.Eukaryotic cell · 2012Article
- Defining pheromone-receptor signaling in Candida albicans and related asexual Candida species.Molecular biology of the cell · 2011Article
- Fungal mating pheromones: choreographing the dating game.Fungal genetics and biology : FG & B · 2011Review
- Strategies for isolating constitutively active and dominant-negative pheromone receptor mutants in yeast.Methods in enzymology · 2010Article
- Chemical gradients and chemotropism in yeast.Cold Spring Harbor perspectives in biology · 2009Review
- Article
- Affinity purification and characterization of a G-protein coupled receptor, Saccharomyces cerevisiae Ste2p.Protein expression and purification · 2007Article
- Article
- The melanosomal/lysosomal protein OA1 has properties of a G protein-coupled receptor.Pigment cell research · 2006Article
- Oligomerization of G-protein-coupled receptors: lessons from the yeast Saccharomyces cerevisiae.Eukaryotic cell · 2005Review
- Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Binding of the alpha-factor pheromone to its G-protein-coupled receptor (encoded by STE2) activates the mating pathway in MATa yeast cells. To investigate whether specific interactions between the receptor and the G protein occur prior to ligand binding, we analyzed dominant-negative mutant receptors that compete with wild-type receptors for G proteins, and we analyzed the ability of receptors to suppress the constitutive signaling activity of mutant Galpha subunits in an alpha-factor-independent manner. Although the amino acid substitution L236H in the third intracellular loop of the receptor impairs G-protein activation, this substitution had no influence on the ability of the dominant-negative receptors to sequester G proteins or on the ability of receptors to suppress the GPA1-A345T mutant Galpha subunit. In contrast, removal of the cytoplasmic C-terminal domain of the receptor eliminated both of these activities even though the C-terminal domain is unnecessary for G-protein activation. Moreover, the alpha-factor-independent signaling activity of ste2-P258L mutant receptors was inhibited by the coexpression of wild-type receptors but not by coexpression of truncated receptors lacking the C-terminal domain. Deletion analysis suggested that the distal half of the C-terminal domain is critical for sequestration of G proteins. The C-terminal domain was also found to influence the affinity of the receptor for alpha-factor in cells lacking G proteins. These results suggest that the C-terminal cytoplasmic domain of the alpha-factor receptor, in addition to its role in receptor downregulation, promotes the formation of receptor-G-protein preactivation complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.