ArticleMolecular and cellular biology2000
Polarized growth controls cell shape and bipolar bud site selection in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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68 citing papers in PubMed, 128 citations in OpenAlex.
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- The Ste20 Homologue FvM4K1 Regulates Organ Size via Hippo Signalling Pathway in Woodland Strawberry (Fragaria vesca).Plant biotechnology journal · 2025Article
- Fission-independent compartmentalization of mitochondria during budding yeast cell division.The Journal of cell biology · 2024Article
- Building a eukaryotic chromosome arm by de novo design and synthesis.Nature communications · 2023Article
- De novo evolution of macroscopic multicellularity.Nature · 2023Article
- Chitin Biosynthesis inJournal of fungi (Basel, Switzerland) · 2023Review
- Systematic Metabolic Profiling IdentifiesmSystems · 2022Article
- The premetazoan ancestry of the synaptic toolkit and appearance of first neurons.Essays in biochemistry · 2022Article
- A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.PloS one · 2022Article
- Gene by Environment Interactions reveal new regulatory aspects of signaling network plasticity.PLoS genetics · 2022Article
- Type V myosin focuses the polarisome and shapes the tip of yeast cells.The Journal of cell biology · 2021Article
- Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Sphingolipids are required for exocyst polarity and exocytic secretion inCell & bioscience · 2020Article
- Aggregate Filamentous Growth Responses in Yeast.mSphere · 2019Article
- Cell-cycle control of cell polarity in yeast.The Journal of cell biology · 2019Article
- Article
- Deciphering the mechanism of action of 089, a compound impairing the fungal cell cycle.Scientific reports · 2018Article
- Cellular packing, mechanical stress and the evolution of multicellularity.Nature physics · 2018Article
- How to bake a brain: yeast as a model neuron.Current genetics · 2016Review
- Three Different Pathways Prevent Chromosome Segregation in the Presence of DNA Damage or Replication Stress in Budding Yeast.PLoS genetics · 2015Article
8 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
Abstract
We examined the relationship between polarized growth and division site selection, two fundamental processes important for proper development of eukaryotes. Diploid Saccharomyces cerevisiae cells exhibit an ellipsoidal shape and a specific division pattern (a bipolar budding pattern). We found that the polarity genes SPA2, PEA2, BUD6, and BNI1 participate in a crucial step of bud morphogenesis, apical growth. Deleting these genes results in round cells and diminishes bud elongation in mutants that exhibit pronounced apical growth. Examination of distribution of the polarized secretion marker Sec4 demonstrates that spa2Delta, pea2Delta, bud6Delta, and bni1Delta mutants fail to concentrate Sec4 at the bud tip during apical growth and at the division site during repolarization just prior to cytokinesis. Moreover, cell surface expansion is not confined to the distal tip of the bud in these mutants. In addition, we found that the p21-activated kinase homologue Ste20 is also important for both apical growth and bipolar bud site selection. We further examined how the duration of polarized growth affects bipolar bud site selection by using mutations in cell cycle regulators that control the timing of growth phases. The grr1Delta mutation enhances apical growth by stabilizing G(1) cyclins and increases the distal-pole budding in diploids. Prolonging polarized growth phases by disrupting the G(2)/M cyclin gene CLB2 enhances the accuracy of bud site selection in wild-type, spa2Delta, and ste20Delta cells, whereas shortening the polarized growth phases by deleting SWE1 decreases the fidelity of bipolar budding. This study reports the identification of components required for apical growth and demonstrates the critical role of polarized growth in bipolar bud site selection. We propose that apical growth and repolarization at the site of cytokinesis are crucial for establishing spatial cues used by diploid yeast cells to position division planes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.