ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Immunoisolation of GABA-specific synaptic vesicles defines a functionally distinct subset of synaptic vesicles.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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Who cites it
70 citing papers in PubMed, 175 citations in OpenAlex.
- Levetiracetam restores GABAergic function and network homeostasis following Vps35 deficiency in excitatory neurons.Translational neurodegeneration · 2026Article
- Ppp2r1a haploinsufficiency increases excitatory synaptic transmission and decreases spatial learning by impairing endocannabinoid signaling.The Journal of clinical investigation · 2025Article
- Coexistence of CN1A autoantibodies in GAD65 encephalitis exacerbates neurodegeneration : Novel autoantibodies in GAD65 encephalitis.Journal of neuroinflammation · 2025Article
- Synaptic vesicle-omics in mice captures signatures of aging and synucleinopathy.Nature communications · 2025Article
- Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading.Science advances · 2025Article
- A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.Current research in physiology · 2024Article
- Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain.Journal of visualized experiments : JoVE · 2022Article
- Rapid and Gentle Immunopurification of Brain Synaptic Vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Co-packaging of opposing neurotransmitters in individual synaptic vesicles in the central nervous system.Neuron · 2022Article
- LAR-RPTPs Directly Interact with Neurexins to Coordinate Bidirectional Assembly of Molecular Machineries.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.The EMBO journal · 2020Article
- Horizontal Cell Feedback to Cone Photoreceptors in Mammalian Retina: Novel Insights From the GABA-pH Hybrid Model.Frontiers in cellular neuroscience · 2020Review
- GABAergic Medial Septal Neurons with Low-Rhythmic Firing Innervating the Dentate Gyrus and Hippocampal Area CA3.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.eLife · 2019Article
- Confocal Synaptology: Synaptic Rearrangements in Neurodegenerative Disorders and upon Nervous System Injury.Frontiers in neuroanatomy · 2018Review
- Synaptic vesicles isolated from the electric organ ofGenomics data · 2017Article
- The dynamics of GABA signaling: Revelations from the circadian pacemaker in the suprachiasmatic nucleus.Frontiers in neuroendocrinology · 2017Review
- Unique pH dynamics in GABAergic synaptic vesicles illuminates the mechanism and kinetics of GABA loading.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Functionally heterogeneous synaptic vesicle pools support diverse synaptic signalling.The Journal of physiology · 2016Review
- Genetic Deletion of the Clathrin Adaptor GGA3 Reduces Anxiety and Alters GABAergic Transmission.PloS one · 2016Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptic vesicles from mammalian brain are among the best characterized trafficking organelles. However, so far it has not been possible to characterize vesicle subpopulations that are specific for a given neurotransmitter. Taking advantage of the recent molecular characterization of vesicular neurotransmitter transporters, we have used an antibody specific for the vesicular GABA transporter (VGAT) to isolate GABA-specific synaptic vesicles. The isolated vesicles are of exceptional purity as judged by electron microscopy. Immunoblotting revealed that isolated vesicles contain most of the major synaptic vesicle proteins in addition to VGAT and are devoid of vesicular monoamine and acetylcholine transporters. The vesicles are 10-fold enriched in GABA uptake activity when compared with the starting vesicle fraction. Furthermore, glutamate uptake activity and glutamate-induced but not chloride-induced acidification are selectively lost during immunoisolation. We conclude that the population of GABA-containing synaptic vesicles is separable and distinct from vesicle populations transporting other neurotransmitters.
Indexed as
Identifiers
10864948PMC6772304W2106255703What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.