ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Bidirectional modulation of exocytosis by angiotensin II involves multiple G-protein-regulated transduction pathways in chromaffin cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
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- Molecular mechanism for muscarinic MThe Journal of physiology · 2017Article
- Arrestin-biased AT1R agonism induces acute catecholamine secretion through TRPC3 coupling.Nature communications · 2017Article
- Muscarinic inhibition of nicotinic transmission in rat sympathetic neurons and adrenal chromaffin cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- Identification of muscarinic receptor subtypes involved in catecholamine secretion in adrenal medullary chromaffin cells by genetic deletion.British journal of pharmacology · 2015Article
- Lactate-mediated glia-neuronal signalling in the mammalian brain.Nature communications · 2014Article
- β1-Blockers Lower Norepinephrine Release by Inhibiting Presynaptic, Facilitating β1-Adrenoceptors in Normotensive and Hypertensive Rats.Frontiers in neurology · 2014Article
- Influence of Fimasartan (a Novel AT(1) Receptor Blocker) on Catecholamine Release in the Adrenal Medulla of Spontaneously Hypertensive Rats.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2013Article
- Peripheral galanin receptor 2 as a target for the modulation of pain.Pain research and treatment · 2012Article
- Activation of the galanin receptor 2 in the periphery reverses nerve injury-induced allodynia.Molecular pain · 2011Article
- Inhibitory effects of olmesartan on catecholamine secretion from the perfused rat adrenal medulla.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2010Article
- Localization of type-2 angiotensin II receptor in adrenal gland.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- The use of viral gene transfer in studies of brainstem noradrenergic and serotonergic neurons.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2009Review
- Effects of losartan on catecholamine release in the isolated rat adrenal gland.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2009Article
- Area-specific differences in transmitter release in central catecholaminergic neurons of spontaneously hypertensive rats.Hypertension (Dallas, Tex. : 1979) · 2008Article
- Differential regulation of endogenous N- and P/Q-type Ca2+ channel inactivation by Ca2+/calmodulin impacts on their ability to support exocytosis in chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Potentiation of exocytosis by phospholipase C-coupled G-protein-coupled receptors requires the priming protein Munc13-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Angiotensin II causes calcium entry into bovine adrenal chromaffin cells via pathway(s) activated by depletion of intracellular calcium stores.Neurochemical research · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Angiotensin II (AngII) receptors couple to a multitude of different types of G-proteins resulting in activation of numerous signaling pathways. In this study we examined the consequences of this promiscuous G-protein coupling on secretion. Chromaffin cells were voltage-clamped at -80 mV in perforated-patch configuration, and Ca(2+)-dependent exocytosis was evoked with brief voltage steps to +20 mV. Vesicle fusion was monitored by changes in membrane capacitance (DeltaC(m)), and released catecholamine was detected with single-cell amperometry. Ca(2+) signaling was studied by recording voltage-dependent Ca(2+) currents (I(Ca)) and by measuring intracellular Ca(2+) ([Ca(2+)](i)) with fura-2 AM. AngII inhibited I(Ca) (IC(50) = 0.3 nm) in a voltage-dependent, pertussis toxin (PTX)-sensitive manner consistent with G(i/o)-protein coupling to Ca(2+) channels. DeltaC(m) was modulated bi-directionally; subnanomolar AngII inhibited depolarization-evoked exocytosis, whereas higher concentrations, in spite of I(Ca) inhibition, potentiated DeltaC(m) fivefold (EC(50) = 3.4 nm). Potentiation of exocytosis by AngII involved activation of phospholipase C (PLC) and Ca(2+) mobilization from internal stores. PTX treatment did not affect AngII-dependent Ca(2+) mobilization or facilitation of exocytosis. However, protein kinase C (PKC) inhibitors decreased the facilitatory effects but not the inhibitory effects of AngII on stimulus-secretion coupling. The AngII type 1 receptor (AT1R) antagonist losartan blocked both inhibition and facilitation of secretion by AngII. The results of this study show that activation of multiple types of G-proteins and transduction pathways by a single neuromodulator acting through one receptor type can produce concentration-dependent, bi-directional regulation of exocytosis.
Indexed as
Identifiers
10864935PMC6772261W1771625224What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.