ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Alternative splicing in the cytoplasmic II-III loop of the N-type Ca channel alpha 1B subunit: functional differences are beta subunit-specific.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Mechanism of gabapentinoid potentiation of opioid effects on cyclic AMP signaling in neuropathic pain.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Tissue- and cell-specific expression of a splice variant in the II-III cytoplasmic loop of Cacna1b.FEBS open bio · 2019Article
- The CaScientific reports · 2018Article
- Central Mechanisms Mediating Thrombospondin-4-induced Pain States.The Journal of biological chemistry · 2016Article
- The Physiology, Pathology, and Pharmacology of Voltage-Gated Calcium Channels and Their Future Therapeutic Potential.Pharmacological reviews · 2015Review
- Calcium Channel CaVα₁ Splice Isoforms - Tissue Specificity and Drug Action.Current molecular pharmacology · 2015Review
- Control of neuronal voltage-gated calcium ion channels from RNA to protein.Trends in neurosciences · 2013Review
- Alternative splicing: functional diversity among voltage-gated calcium channels and behavioral consequences.Biochimica et biophysica acta · 2013Review
- Opioid inhibition of N-type Ca2+ channels and spinal analgesia couple to alternative splicing.Nature neuroscience · 2010Article
- Regulation of N-type voltage-gated calcium channels (Cav2.2) and transmitter release by collapsin response mediator protein-2 (CRMP-2) in sensory neurons.Journal of cell science · 2009Article
- Alternative splicing in the synaptic protein interaction site of rat Ca(v)2.2 (alpha (1B)) calcium channels: changes induced by chronic inflammatory pain.Journal of molecular neuroscience : MN · 2009Article
- Differential role of N-type calcium channel splice isoforms in pain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Dehydration increases L-type Ca(2+) current in rat supraoptic neurons.The Journal of physiology · 2007Article
- Ziconotide: a review of its pharmacology and use in the treatment of pain.Neuropsychiatric disease and treatment · 2007Article
- Developmentally regulated actions of alcohol on hippocampal glutamatergic transmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Cumulative inactivation of N-type CaV2.2 calcium channels modified by alternative splicing.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The impact of splice isoforms on voltage-gated calcium channel alpha1 subunits.The Journal of physiology · 2004Review
- Functional diversity in neuronal voltage-gated calcium channels by alternative splicing of Ca(v)alpha1.Molecular neurobiology · 2002Review
- Synaptic calcium-channel function in Drosophila: analysis and transformation rescue of temperature-sensitive paralytic and lethal mutations of cacophony.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Structural diversity of voltage-gated Ca channels underlies much of the functional diversity in Ca signaling in neurons. Alternative splicing is an important mechanism for generating structural variants within a single gene family. In this paper, we show the expression pattern of an alternatively spliced 21 amino acid encoding exon in the II-III cytoplasmic loop region of the N-type Ca channel alpha(1B) subunit and assess its functional impact. Exon-containing alpha(1B) mRNA dominated in sympathetic ganglia and was present in approximately 50% of alpha(1B) mRNA in spinal cord and caudal regions of the brain and in the minority of alpha(1B) mRNA in neocortex, hippocampus, and cerebellum (<20%). The II-III loop exon affected voltage-dependent inactivation of the N-type Ca channel. Steady-state inactivation curves were shifted to more depolarized potentials without affects on either the rate or voltage dependence of channel opening. Differences in voltage-dependent inactivation between alpha(1B) splice variants were most clearly manifested in the presence of Ca channel beta(1b) or beta(4), rather than beta(2a) or beta(3), subunits. Our results suggest that exon-lacking alpha(1B) splice variants that associate with beta(1b) and beta(4) subunits will be susceptible to voltage-dependent inactivation at voltages in the range of neuronal resting membrane potentials (-60 to -80 mV). In contrast, alpha(1B) splice variants that associate with either beta(2a) or beta(3) subunits will be relatively resistant to inactivation at these voltages. The potential to mix and match multiple alpha(1B) splice variants and beta subunits probably represents a mechanism for controlling the plasticity of excitation-secretion coupling at different synapses.
Indexed as
Identifiers
10864934PMC6772276W2096395994What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.