ArticleJournal of virology2000
Characterization of a baculovirus alkaline nuclease.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Reshaping of the substrate-binding pocket to improve the catalytic activity of the non-specific endonuclease fromFrontiers in bioengineering and biotechnology · 2026Article
- Cloning, heterologous expression, and molecular characterization of a highly active and stable non-specific endonuclease from Pseudomonas fluorescens.Archives of microbiology · 2024Article
- Bacterial non-specific nucleases of the phospholipase D superfamily and their biotechnological potential.Applied microbiology and biotechnology · 2020Review
- Characterization of Single Amino Acid Variations in an EDTA-Tolerating Non-specific Nuclease from the Ice-Nucleating Bacterium Pseudomonas syringae.Molecular biotechnology · 2020Article
- Cidofovir Diphosphate Inhibits Adenovirus 5 DNA Polymerase via both Nonobligate Chain Termination and Direct Inhibition, and Polymerase Mutations Confer Cidofovir Resistance on Intact Virus.Antimicrobial agents and chemotherapy · 2019Article
- Global Analysis of Baculovirus Autographa californica Multiple Nucleopolyhedrovirus Gene Expression in the Midgut of the Lepidopteran Host Trichoplusia ni.Journal of virology · 2018Article
- Analysis of the autographa californica multiple nucleopolyhedrovirus overlapping gene pair lef3 and ac68 reveals that AC68 is a per os infectivity factor and that LEF3 is critical, but not essential, for virus replication.Journal of virology · 2012Article
- Analysis of the genome of the sexually transmitted insect virus Helicoverpa zea nudivirus 2.Viruses · 2012Article
- Structural and functional insight into the mechanism of an alkaline exonuclease from Laribacter hongkongensis.Nucleic acids research · 2011Article
- Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae.BMC molecular biology · 2011Article
- Epstein-Barr virus DNase (BGLF5) induces genomic instability in human epithelial cells.Nucleic acids research · 2010Article
- Isolation and characterization of the DNA-binding protein (DBP) of the Autographa californica multiple nucleopolyhedrovirus.Virology · 2008Article
- A baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids.Virology · 2007Article
- The baculoviruses occlusion-derived virus: virion structure and function.Advances in virus research · 2007Review
- Characterization of the role of very late expression factor 1 in baculovirus capsid structure and DNA processing.Journal of virology · 2006Article
- Characterization of a baculovirus lacking the alkaline nuclease gene.Journal of virology · 2004Article
- Sequence analysis of the genome of the Neodiprion sertifer nucleopolyhedrovirus.Journal of virology · 2004Article
- Baculovirus alkaline nuclease possesses a 5'-->3' exonuclease activity and associates with the DNA-binding protein LEF-3.Journal of virology · 2003Article
- Genetic requirements for homologous recombination in Autographa californica nucleopolyhedrovirus.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All baculovirus genomes sequenced to date encode a homolog of an alkaline nuclease that has been characterized in the Herpesviridae. In this report we describe the characterization of the alkaline nuclease (AN) homolog of the Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) (open reading frame 133). His-tagged AN constructs were expressed in recombinant baculoviruses and affinity purified, and then their enzymatic activity was characterized. AN was found to degrade linear DNA at alkaline pH, preferred Mg(2+) over Mn(2+), had optimal activity at 35 degrees C, and did not appear to have a salt requirement. To rule out contamination by the endogenous baculovirus gene product or a cellular enzyme, point mutations were introduced into a highly conserved domain of the gene. These mutations were found to markedly reduce or eliminate most of the activity of the affinity-purified enzyme. An antibody generated against the protein was used to analyze its expression by Western blot analysis. AN was found to be expressed at low levels by 12 h postinfection, with maximal expression at 24 h postinfection. Attempts to generate a virus with this gene inactivated were unsuccessful, suggesting that AN may be encoded by an essential gene.
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