ArticleThe Biochemical journal2000
Inhibition of Ca(2+) signalling by p130, a phospholipase-C-related catalytically inactive protein: critical role of the p130 pleckstrin homology domain.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow.Scientific reports · 2019Article
- Suppression of cell migration by phospholipase C-related catalytically inactive protein-dependent modulation of PI3K signalling.Scientific reports · 2017Article
- Phospholipase C-related but catalytically inactive proteins regulate ovarian follicle development.The Journal of biological chemistry · 2017Article
- Phospholipase C-related, but catalytically inactive protein (PRIP) up-regulates osteoclast differentiation via calcium-calcineurin-NFATc1 signaling.The Journal of biological chemistry · 2017Article
- Phospholipase C-related catalytically inactive protein can regulate obesity, a state of peripheral inflammation.The Japanese dental science review · 2017Review
- Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.Biology open · 2014Article
- Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- PRIP (phospholipase C-related but catalytically inactive protein) inhibits exocytosis by direct interactions with syntaxin 1 and SNAP-25 through its C2 domain.The Journal of biological chemistry · 2013Article
- Phospholipase C-related but catalytically inactive protein (PRIP) modulates synaptosomal-associated protein 25 (SNAP-25) phosphorylation and exocytosis.The Journal of biological chemistry · 2012Article
- Involvement of PRIP, phospholipase C-related, but catalytically inactive protein, in bone formation.The Journal of biological chemistry · 2011Article
- Calcium signalling in early embryos.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- phospholipase C, beta 3 is required for Endothelin1 regulation of pharyngeal arch patterning in zebrafish.Developmental biology · 2007Article
- Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- Microdomains bounded by endoplasmic reticulum segregate cell cycle calcium transients in syncytial Drosophila embryos.The Journal of cell biology · 2005Article
- Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channels.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- GABAA receptor phospho-dependent modulation is regulated by phospholipase C-related inactive protein type 1, a novel protein phosphatase 1 anchoring protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Co-incident signalling between mu-opioid and M3 muscarinic receptors at the level of Ca2+ release from intracellular stores: lack of evidence for Ins(1,4,5)P3 receptor sensitization.The Biochemical journal · 2003Article
- Molecular modeling of the membrane targeting of phospholipase C pleckstrin homology domains.Protein science : a publication of the Protein Society · 2003Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
p130 was originally identified as an Ins(1,4,5)P(3)-binding protein similar to phospholipase C-delta but lacking any phospholipase activity. In the present study we have further analysed the interactions of p130 with inositol compounds in vitro. To determine which of the potential ligands interacts with p130 in cells, we performed an analysis of the cellular localization of this protein, the isolation of a protein-ligand complex from cell lysates and studied the effects of p130 on Ins(1,4,5)P(3)-mediated Ca(2+) signalling by using permeabilized and transiently or stably transfected COS-1 cells (COS-1(p130)). In vitro, p130 bound Ins(1,4,5)P(3) with a higher affinity than that for phosphoinositides. When the protein was isolated from COS-1(p130) cells by immunoprecipitation, it was found to be associated with Ins(1,4,5)P(3). Localization studies demonstrated the presence of the full-length p130 in the cytoplasm of living cells, not at the plasma membrane. In cell-based assays, p130 had an inhibitory effect on Ca(2+) signalling. When fura-2-loaded COS-1(p130) cells were stimulated with bradykinin, epidermal growth factor or ATP, it was found that the agonist-induced increase in free Ca(2+) concentration, observed in control cells, was inhibited in COS-1(p130). This inhibition was not accompanied by the decreased production of Ins(1,4,5)P(3); the intact p130 pleckstrin homology domain, known to be the ligand-binding site in vitro, was required for this effect in cells. These results suggest that Ins(1,4,5)P(3) could be the main p130 ligand in cells and that this binding has the potential to inhibit Ins(1,4,5)P(3)-mediated Ca(2+) signalling.
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