ArticleThe Biochemical journal2000
Pyruvate dehydrogenase kinase from Arabidopsis thaliana: a protein histidine kinase that phosphorylates serine residues.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- Regulation of plant glycolysis and the tricarboxylic acid cycle by posttranslational modifications.The Plant journal : for cell and molecular biology · 2025Review
- Genome-wide identification and functional characterization of the two-component system gene family in petunia reveals roles in hormone signaling and stress response.Frontiers in plant science · 2025Article
- An evolutionarily conserved iron-sulfur cluster underlies redox sensory function of the Chloroplast Sensor Kinase.Communications biology · 2020Article
- Phosphorylation of PhosphoThe Plant cell · 2017Article
- Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts.Photosynthesis research · 2016Article
- Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.PloS one · 2016Article
- Posttranslational Protein Modifications in Plant Metabolism.Plant physiology · 2015Article
- Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum.Microbial cell factories · 2014Article
- Biochemistry, proteomics, and phosphoproteomics of plant mitochondria from non-photosynthetic cells.Frontiers in plant science · 2013Article
- ABC1K atypical kinases in plants: filling the organellar kinase void.Trends in plant science · 2012Review
- Intrinsic negative feedback governs activation surge in two-component regulatory systems.Molecular cell · 2012Article
- Cloning and functions analysis of a pyruvate dehydrogenase kinase in Brassica napus.Plant cell reports · 2011Article
- Mitochondrial biogenesis and function in Arabidopsis.The arabidopsis book · 2008Article
- Two-component signaling elements and histidyl-aspartyl phosphorelays.The arabidopsis book · 2008Article
- Branched-chain 6-ketoacid dehydrogenase kinase: A mammalian enzyme with histidine kinase activity.Journal of biomolecular techniques : JBT · 2002Article
- Structure of rat BCKD kinase: nucleotide-induced domain communication in a mitochondrial protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- An essential role of Glu-243 and His-239 in the phosphotransfer reaction catalyzed by pyruvate dehydrogenase kinase.The Journal of biological chemistry · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyruvate dehydrogenase kinase (PDK) is the primary regulator of flux through the mitochondrial pyruvate dehydrogenase complex (PDC). Although PDKs inactivate mitochondrial PDC by phosphorylating specific Ser residues, the primary amino acid sequence indicates that they are more closely related to prokaryotic His kinases than to eukaryotic Ser/Thr kinases. Unlike Ser/Thr kinases, His kinases use a conserved His residue for phosphotransfer to Asp residues. To understand these unique kinases better, a presumptive PDK from Arabidopsis thaliana was heterologously expressed and purified for this investigation. Purified, recombinant A. thaliana PDK could inactivate kinase-depleted maize mitochondrial PDC by phosphorylating Ser residues. Additionally, A. thaliana PDK was capable of autophosphorylating Ser residues near its N-terminus, although this reaction is not part of the phosphotransfer pathway. To elucidate the mechanism involved, we performed site-directed mutagenesis of the canonical His residue likely to be involved in phosphotransfer. When His-121 was mutated to Ala or Gln, Ser-autophosphorylation was decreased by 50% and transphosphorylation of PDC was decreased concomitantly. We postulate that either (1) His-121 is not the sole phosphotransfer His residue or (2) mutagenesis of His-121 exposes an additional otherwise cryptic phosphotransfer His residue. Thus His-121 is one residue involved in kinase function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.