ArticleThe Biochemical journal2000
Regulation of sterol regulatory-element binding protein 1 gene expression in liver: role of insulin and protein kinase B/cAkt.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 185 citations in OpenAlex.
- A novel JNK2/SREBP-1c pathway involved in insulin-induced fatty acid synthesis in human adipocytes.Journal of lipid research · 2013Trial
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- Liver regeneration: cytokine regulation targeting hepatocytes and beyond.Life medicine · 2026Review
- The role of fibroblast growth factors in cell and cancer metabolism.FEBS letters · 2026Review
- Metabolic stress-induced human beta-cell death is mediated by increased intracellular levels of adenosine.Frontiers in endocrinology · 2023Article
- Dyslipidemia in Children Treated with a BRAF Inhibitor for Low-Grade Gliomas: A New Side Effect?Cancers · 2022Article
- INPP4B protects from metabolic syndrome and associated disorders.Communications biology · 2021Article
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- Extracellular Matrix Stiffness: New Areas Affecting Cell Metabolism.Frontiers in oncology · 2021Review
- The balance of protein farnesylation and geranylgeranylation during the progression of nonalcoholic fatty liver disease.The Journal of biological chemistry · 2020Review
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- The kinase PKD3 provides negative feedback on cholesterol and triglyceride synthesis by suppressing insulin signaling.Science signaling · 2019Article
- Yarrow supercritical extract exerts antitumoral properties by targeting lipid metabolism in pancreatic cancer.PloS one · 2019Article
- OSBP-related protein-2 (ORP2): a novel Akt effector that controls cellular energy metabolism.Cellular and molecular life sciences : CMLS · 2018Article
- PHD3 regulates glucose metabolism by suppressing stress-induced signalling and optimising gluconeogenesis and insulin signalling in hepatocytes.Scientific reports · 2018Article
- Sustained SREBP-1-dependent lipogenesis as a key mediator of resistance to BRAF-targeted therapy.Nature communications · 2018Article
- Article
- POSSIBLE REGULATION OF LDL-RECEPTOR BY NARINGENIN IN HEPG2 HEPATOMA CELL LINE.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2017Article
- The multifaceted roles of fatty acid synthesis in cancer.Nature reviews. Cancer · 2016Review
- The interplay between cell signalling and the mevalonate pathway in cancer.Nature reviews. Cancer · 2016Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulin stimulates the transcription of the sterol regulatory- element binding protein (SREBP) 1/ADD1 gene in liver. Hepatocytes in primary culture were used to delineate the insulin signalling pathway for induction of SREBP1 gene expression. The inhibitors of phosphoinositide 3-kinase (PI 3-kinase), wortmannin and LY 294002, abolished the insulin-dependent increase in SREBP1 mRNA, whereas the inhibitor of the mitogen- activated protein kinase cascade, PD 98059, was without effect. To investigate the role of protein kinase B (PKB)/cAkt downstream of PI 3-kinase, hepatocytes were transduced with an adenovirus encoding a PKB--oestrogen receptor fusion protein. The PKB activity of this recombinant protein was rapidly activated in hepatocytes challenged with 4-hydroxytamoxifen (OHT), as was endogenous PKB in hepatocytes challenged with insulin. The addition of OHT to transduced hepatocytes resulted in accumulation of SREBP1 mRNA, with a time-course and magnitude similar to the effect of insulin in non-transduced cells. The level of SREBP1 mRNA was not increased by OHT in hepatocytes expressing a mutant form of the recombinant protein whose PKB activity was not activated by OHT. Thus acute activation of PKB is sufficient to induce SREBP1 mRNA accumulation in primary hepatocytes, and might be the major signalling event by which insulin induces SREBP1 gene expression in the liver.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.