ArticleProceedings of the National Academy of Sciences of the United States of America2000
Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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Who cites it
66 citing papers in PubMed.
- The physicochemical design, structure and permeability of the JCVI-syn3A minimal cell membrane.Nature communications · 2026Article
- Salt supplementation-induced metabolic reprogramming inmSystems · 2026Article
- Developing Effective Bioinoculant and Engineering Plant Microbiome for Climate Resilient Agriculture: Lessons Learned and Future Roadmap.Current microbiology · 2026Review
- Nutritional pressure from serum amplifies dysbiotic features in an oral microbiome synthetic community.The ISME journal · 2026Article
- Molecular signatures and machine learning driven stress biomarkers for rainbow trout aquaculture and climate adaptation.Scientific reports · 2025Article
- The Impact of Serum on a Complex Synthetic Community Model of the Subgingival Microbiome.bioRxiv : the preprint server for biology · 2025Article
- Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.STAR protocols · 2025Article
- A Closer Look at the Potential Mechanisms of Action of Protective Agents Used in the Drying of Microorganisms: A Review.Microorganisms · 2025Review
- Comparative genomics and characterization of highly adhesive and stress resistantCurrent research in food science · 2025Article
- Exploring the Ligand Binding and Conformational Dynamics of the Substrate-Binding Domain 1 of the ABC Transporter GlnPQ.The journal of physical chemistry. B · 2024Article
- The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact.eLife · 2024Article
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- Effects of High Temperature-Triggered Transcriptomics on the Physiological Adaptability ofMicroorganisms · 2022Article
- Streptococcus suis TrpX is part of a tryptophan uptake system, and its expression is regulated by a T-box regulatory element.Scientific reports · 2022Article
- Comparative Transcriptomics and Gene Knockout Reveal Virulence Factors ofFrontiers in microbiology · 2022Article
- Survival characteristics and transcriptome profiling reveal the adaptive response of theFrontiers in microbiology · 2022Article
- Role of RhizosphericFrontiers in plant science · 2022Article
- Heterodimer Formation of the Homodimeric ABC Transporter OpuA.International journal of molecular sciences · 2021Article
- Single Cell-like Systems Reveal Active Unidirectional and Light-Controlled Transport by Nanomachineries.ACS nano · 2021Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An osmoregulated ABC transporter (OpuA) with novel structural features has been identified that responds to water stress. This glycine betaine transport system consists of an ATP-binding/hydrolyzing subunit (OpuAA) and a protein (OpuABC) that contains both the translocator and the substrate-binding domain. The components of OpuA have been overexpressed, purified, and functionally incorporated into liposomes with an ATP-regenerating system in the vesicle lumen. A transmembrane osmotic gradient (outside hyperosmotic relative to the inside) of both ionic and nonionic compounds was able to osmotically activate OpuA in the proteoliposomal system. Hypoosmotic medium conditions inhibited the basal activity of the system. The data show that OpuAA and OpuABC are sufficient for osmoregulated transport, indicating that OpuA can act both as osmosensor and osmoregulator. Strikingly, OpuA could also be activated by low concentrations of cationic and anionic amphipaths, which interact with the membrane. This result indicates that activation by a transmembrane osmotic gradient is mediated by changes in membrane properties/protein-lipid interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.