ArticlePlant physiology2000
Calcium-calmodulin suppresses the filamentous actin-binding activity of a 135-kilodalton actin-bundling protein isolated from lily pollen tubes.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 82 citations in OpenAlex.
- Local anesthetic lidocaine decelerates cytoplasmic streaming inPlant signaling & behavior · 2026Article
- Actin Bundles in The Pollen Tube.International journal of molecular sciences · 2018Review
- Article
- Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.Nature communications · 2016Article
- The Cytoskeleton and Its Regulation by Calcium and Protons.Plant physiology · 2016Review
- Interaction between Calcium and Actin in Guard Cell and Pollen Signaling Networks.Plants (Basel, Switzerland) · 2013Review
- MAP18 regulates the direction of pollen tube growth in Arabidopsis by modulating F-actin organization.The Plant cell · 2013Article
- FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe.The Plant cell · 2012Article
- Bundling actin filaments from membranes: some novel players.Frontiers in plant science · 2012Article
- Arabidopsis VILLIN5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth.The Plant cell · 2010Article
- Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover.The Plant cell · 2010Article
- Calcium participates in feedback regulation of the oscillating ROP1 Rho GTPase in pollen tubes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Analysis of the tip-to-base gradient of CaM in pollen tube pulsant growth using in vivo CaM-GFP system.Plant cell reports · 2009Article
- Combined proteomic and cytological analysis of Ca2+-calmodulin regulation in Picea meyeri pollen tube growth.Plant physiology · 2009Article
- An actin-binding protein, LlLIM1, mediates calcium and hydrogen regulation of actin dynamics in pollen tubes.Plant physiology · 2008Article
- Pollen tube growth oscillations and intracellular calcium levels are reversibly modulated by actin polymerization.Plant physiology · 2008Article
- Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination.Plant physiology · 2007Article
- The sliding theory of cytoplasmic streaming: fifty years of progress.Journal of plant research · 2007Review
- Signalling pathways in pollen germination and tube growth.Protoplasma · 2006Review
- Modulation of endocytosis in pollen tube growth by phosphoinositides and phospholipids.Protoplasma · 2005Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated a 135-kD actin-bundling protein (P-135-ABP) from lily (Lilium longiflorum) pollen tubes and have shown that this protein is responsible for bundling actin filaments in lily pollen tubes (E. Yokota, K. Takahara, T. Shimmen [1998] Plant Physiol 116: 1421-1429). However, only a few thin actin-filament bundles are present in random orientation in the tip region of pollen tubes, where high concentrations of Ca(2+) have also been found. To elucidate the molecular mechanism for the temporal and spatial regulation of actin-filament organization in the tip region of pollen tubes, we explored the possible presence of factors modulating the filamentous actin (F-actin)-binding activity of P-135-ABP. The F-actin-binding activity of P-135-ABP in vitro was appreciably reduced by Ca(2+) and calmodulin (CaM), although neither Ca(2+) alone nor CaM in the presence of low concentrations of Ca(2+) affects the activity of P-135-ABP. A micromolar order of Ca(2+) and CaM were needed to induce the inhibition of the binding activity of P-135-ABP to F-actin. An antagonist for CaM, W-7, cancelled this inhibition. W-5 also alleviated the inhibition effect of Ca(2+)-CaM, however, more weakly than W-7. These results suggest the specific interaction of P-135-ABP with Ca(2+)-CaM. In the presence of both Ca(2+) and CaM, P-135-ABP organized F-actin into thin bundles, instead of the thick bundles observed in the absence of CaM. These results suggest that the inhibition of the P-135-ABP activity by Ca(2+)-CaM is an important regulatory mechanism for organizing actin filaments in the tip region of lily pollen tubes.
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