ArticlePlant physiology2000
Immunolocalization of a cysteine protease in vacuoles, vesicles, and symbiosomes of pea nodule cells.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 73 citations in OpenAlex.
- Uclacyanin MtUC1 Is Involved in the Regulation of Nodule Senescence in Medicago truncatula.Molecular plant pathology · 2025Article
- Loss of Lateral suppressor gene is associated with evolution of root nodule symbiosis in Leguminosae.Genome biology · 2024Article
- GmNAC039 and GmNAC018 activate the expression of cysteine protease genes to promote soybean nodule senescence.The Plant cell · 2023Article
- Characteristics and Research Progress of Legume Nodule Senescence.Plants (Basel, Switzerland) · 2021Review
- Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis.BMC plant biology · 2020Article
- Transcription Factor bHLH2 RepressesPlant physiology · 2019Article
- Transcriptome profiling illustrates expression signatures of dehydration tolerance in developing grasspea seedlings.Planta · 2019Article
- Purification and characterization of cysteine protease from miswak Salvadora persica.BMC biochemistry · 2018Article
- The Intracellular Localization of the Vanillin Biosynthetic Machinery in Pods of Vanilla planifolia.Plant & cell physiology · 2018Article
- The Symbiosome: Legume and Rhizobia Co-evolution toward a Nitrogen-Fixing Organelle?Frontiers in plant science · 2017Review
- Search for Nodulation and Nodule Development-Related Cystatin Genes in the Genome of Soybean (Frontiers in plant science · 2016Article
- Possible Role of 1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase Activity of Sinorhizobium sp. BL3 on Symbiosis with Mung Bean and Determinate Nodule Senescence.Microbes and environments · 2015Article
- Cysteine protease and cystatin expression and activity during soybean nodule development and senescence.BMC plant biology · 2014Article
- Multiple domains in MtENOD8 protein including the signal peptide target it to the symbiosome.Plant physiology · 2012Article
- Endocytosis in plant-microbe interactions.Protoplasma · 2010Review
- Characterization of a novel organelle in Toxoplasma gondii with similar composition and function to the plant vacuole.Molecular microbiology · 2010Article
- Comparison of developmental and stress-induced nodule senescence in Medicago truncatula.Plant physiology · 2010Article
- Medicago N2-fixing symbiosomes acquire the endocytic identity marker Rab7 but delay the acquisition of vacuolar identity.The Plant cell · 2009Article
- Transcriptome profiling of Lotus japonicus roots during arbuscular mycorrhiza development and comparison with that of nodulation.DNA research : an international journal for rapid publication of reports on genes and genomes · 2007Article
- Aging in legume symbiosis. A molecular view on nodule senescence in Medicago truncatula.Plant physiology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PsCYP15A is a cysteine protease from pea (Pisum sativum L.). It was first recognized as an up-regulated transcript in wilted shoots and subsequently in root nodules containing Rhizobium. Proteolytic activity of PsCYP15A in nodule extracts is now reported following immunopurification with polyclonal antiserum raised against recombinant antigen. Western-blot analysis indicated two forms of PsCYP15A, a pro-form (approximately 38 kD) and a mature form (approximately 30 kD). Both forms were present in most tissue samples, but only the mature form was isolated from cell-fractionated symbiosomes containing nitrogen-fixing bacteroids. Immunolabeling of nodule sections showed localization of PsCYP15A antigen in large vacuolar bodies, cytoplasmic vesicles, and the perisymbiont space. Immunolabeling of tissue sections from wilted shoots also indicated the presence of PsCYP15A in vacuoles and cytoplasmic vesicles. This protease may be involved in the adaptation to changes in cell turgor, both in wilted shoots and in nodule tissue. Additionally, the protease may be involved in protein turnover in the symbiosome compartment.
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Registered trials
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