ArticleInfection and immunity2000
The most abundant glycoprotein of amebic cyst walls (Jacob) is a lectin with five Cys-rich, chitin-binding domains.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Trafficking and organization of cyst wall components into a robust biphasic structure in Entamoeba.PLoS pathogens · 2026Article
- Targets for the diagnosis ofmSphere · 2025Article
- Novel Insights into the Wattle and Daub Model ofPathogens (Basel, Switzerland) · 2023Article
- In Silico Identification and Characterization of circRNAs as Potential Virulence-Related miRNA/siRNA Sponges fromNon-coding RNA · 2022Article
- Article
- The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba.Molecular biology and evolution · 2021Article
- Morphological and Motility Features of the Stable Bleb-Driven Monopodial Form ofInfection and immunity · 2020Article
- The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls.PLoS neglected tropical diseases · 2019Article
- Demonstration and Characterization of Cyst-Like Structures in the Life Cycle ofFrontiers in cellular and infection microbiology · 2019Article
- Membrane Trafficking Modulation during Entamoeba Encystation.Scientific reports · 2017Article
- Entamoeba Encystation: New Targets to Prevent the Transmission of Amebiasis.PLoS pathogens · 2016Review
- Species-Specific Immunodetection of an Entamoeba histolytica Cyst Wall Protein.PLoS neglected tropical diseases · 2016Article
- Ethanol and isopropanol in concentrations present in hand sanitizers sharply reduce excystation of Giardia and Entamoeba and eliminate oral infectivity of Giardia cysts in gerbils.Antimicrobial agents and chemotherapy · 2015Article
- Strategies to discover the structural components of cyst and oocyst walls.Eukaryotic cell · 2013Review
- Different structure and mRNA expression of Entamoeba invadens chitinases in the encystation and excystation.Parasitology research · 2011Article
- A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia.Trends in parasitology · 2011Article
- Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer.PLoS pathogens · 2010Article
- The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic.PLoS neglected tropical diseases · 2010Article
- Selective condensation drives partitioning and sequential secretion of cyst wall proteins in differentiating Giardia lamblia.PLoS pathogens · 2010Article
- Involvement of serine proteases in the excystation and metacystic development of Entamoeba invadens.Parasitology research · 2009Article
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7 authors.
Funding
Abstract
The infectious stage of amebae is the chitin-walled cyst, which is resistant to stomach acids. In this study an extraordinarily abundant, encystation-specific glycoprotein (Jacob) was identified on two-dimensional protein gels of cyst walls purified from Entamoeba invadens. Jacob, which was acidic and had an apparent molecular mass of approximately 100 kDa, contained sugars that bound to concanavalin A and ricin. The jacob gene encoded a 45-kDa protein with a ladder-like series of five Cys-rich domains. These Cys-rich domains were reminiscent of but not homologous to the Cys-rich chitin-binding domains of insect chitinases and peritrophic matrix proteins that surround the food bolus in the insect gut. Jacob bound purified chitin and chitin remaining in sodium dodecyl sulfate-treated cyst walls. Conversely, the E. histolytica plasma membrane Gal/GalNAc lectin bound sugars of intact cyst walls and purified Jacob. In the presence of galactose, E. invadens formed wall-less cysts, which were quadranucleate and contained Jacob and chitinase (another encystation-specific protein) in secretory vesicles. A galactose lectin was found to be present on the surface of wall-less cysts, which phagocytosed bacteria and mucin-coated beads. These results suggest that the E. invadens cyst wall forms when the plasma membrane galactose lectin binds sugars on Jacob, which in turn binds chitin via its five chitin-binding domains.
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