ArticleInfection and immunity2000
Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 162 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
162 citing papers in PubMed, 347 citations in OpenAlex.
- Genetic Diversity of Cryptosporidium in Children in an Urban Informal Settlement of Nairobi, Kenya.PloS one · 2015Trial
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- Occurrence and Genetic Diversity ofAnimals : an open access journal from MDPI · 2026Article
- A Real-Time PCR-Based Method for the Sequence Determination of Cryptosporidium hominis and Cryptosporidium parvum gp60 Subtypes During Epidemiological Investigations.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- Investigating within-host population diversity of Cryptosporidium parvum using BlooMine.BMC genomics · 2025Article
- Genetic crosses reveal genomic loci responsible for virulence in Cryptosporidium parvum infection.Cell reports · 2025Article
- Long-term molecular surveillance of Cryptosporidium and Giardia in wildlife in protected drinking water catchments.Parasites & vectors · 2025Article
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- Deciphering a cryptic minefield: A guide toCurrent research in parasitology & vector-borne diseases · 2025Review
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- Microphysiological gut-on-chip enables extendedFrontiers in cellular and infection microbiology · 2025Article
- Parapipe: a pipeline for parasite next-generation sequencing data analysis applied to Cryptosporidium.Access microbiology · 2025Article
- Investigating genetic diversity withinCurrent research in parasitology & vector-borne diseases · 2025Article
- Design, development, and testing of a new multi-locus sequence typing scheme for the zoonotic pathogenCurrent research in parasitology & vector-borne diseases · 2025Article
- A Pumpless and Tubeless Microfluidic Device Enables Extended In Vitro Development ofOpen forum infectious diseases · 2024Article
102 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The apicomplexan parasite Cryptosporidium parvum is a major cause of serious diarrheal disease in both humans and animals. No efficacious chemo- or immunotherapies have been identified for cryptosporidiosis, but certain antibodies directed against zoite surface antigens and/or proteins shed by gliding zoites have been shown to neutralize infectivity in vitro and/or to passively protect against, or ameliorate, disease in vivo. We previously used monoclonal antibody 11A5 to identify a 15-kDa surface glycoprotein that was shed behind motile sporozoites and was recognized by several lectins that neutralized parasite infectivity for cultured epithelial cells. Here we report the cloning and sequence analysis of the gene encoding this 11A5 antigen. Surprisingly, the gene encoded a 330-amino-acid, mucin-like glycoprotein that was predicted to contain an N-terminal signal peptide, a homopolymeric tract of serine residues, 36 sites of O-linked glycosylation, and a hydrophobic C-terminal peptide specifying attachment of a glycosylphosphatidylinositol anchor. The single-copy gene lacked introns and was expressed during merogony to produce a 60-kDa precursor which was proteolytically cleaved to 15- and 45-kDa glycoprotein products that both localized to the surface of sporozoites and merozoites. The gp15/45/60 gene displayed a very high degree of sequence diversity among C. parvum isolates, and the numerous single-nucleotide and single-amino-acid polymorphisms defined five to six allelic classes, each characterized by additional intra-allelic sequence variation. The gp15/45/60 single-nucleotide polymorphisms will prove useful for haplotyping and fingerprinting isolates and for establishing meaningful relationships between C. parvum genotype and phenotype.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.