ArticleInfection and immunity2000
Molecular cloning and expression of a gene encoding Cryptosporidium parvum glycoproteins gp40 and gp15.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed, 177 citations in OpenAlex.
- Genetic Diversity of Cryptosporidium in Children in an Urban Informal Settlement of Nairobi, Kenya.PloS one · 2015Trial
- Molecular Prevalence andAnimals : an open access journal from MDPI · 2026Article
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- Pathogenicity and virulence ofVirulence · 2025Review
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- Genetic crosses reveal genomic loci responsible for virulence in Cryptosporidium parvum infection.Cell reports · 2025Article
- Cryptosporidiosis: A global threat to human and animal health.Open veterinary journal · 2025Review
- Variant surface protein GP60 contributes to host infectivity of Cryptosporidium parvum.Communications biology · 2024Article
- Identification of host protein ENO1 (alpha-enolase) interacting with Cryptosporidium parvum sporozoite surface protein, Cpgp40.Parasites & vectors · 2024Article
- Stable expression of mucin glycoproteins GP40 and GP15 of Cryptosporidium parvum in Toxoplasma gondii.Parasites & vectors · 2024Article
- Identification of the glycopeptide epitope recognized by a protectiveInfection and immunity · 2023Article
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- Cryptosporidiosis: From Prevention to Treatment, a Narrative Review.Microorganisms · 2022Review
- Development and Application of aMicroorganisms · 2021Article
- Development of a Subtyping Tool for Zoonotic PathogenJournal of clinical microbiology · 2021Article
- Serum IgG Responses to gp15 and gp40 Protein-Derived Synthetic Peptides FromFrontiers in cellular and infection microbiology · 2021Article
- Review
- Interaction between human mucins and parasite glycoproteins: the role of lectins and glycosidases in colonization by intestinal protozoa.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Article
- Differential Expression of ThreeFrontiers in microbiology · 2019Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Cryptosporidium parvum is a significant cause of diarrheal disease worldwide. The specific molecules that mediate C. parvum-host cell interactions and the molecular mechanisms involved in the pathogenesis of cryptosporidiosis are unknown. In this study we have shown that gp40, a mucin-like glycoprotein, is localized to the surface and apical region of invasive stages of the parasite and is shed from its surface. gp40-specific antibodies neutralize infection in vitro, and native gp40 binds specifically to host cells, implicating this glycoprotein in C. parvum attachment to and invasion of host cells. We have cloned and sequenced a gene designated Cpgp40/15 that encodes gp40 as well as gp15, an antigenically distinct, surface glycoprotein also implicated in C. parvum-host cell interactions. Analysis of the deduced amino acid sequence of the 981-bp Cpgp40/15 revealed the presence of an N-terminal signal peptide, a polyserine domain, multiple predicted O-glycosylation sites, a single potential N-glycosylation site, and a hydrophobic region at the C terminus, a finding consistent with what is required for the addition of a GPI anchor. There is a single copy of Cpgp40/15 in the C. parvum genome, and this gene does not contain introns. Our data indicate that the two Cpgp40/15-encoded proteins, gp40 and gp15, are products of proteolytic cleavage of a 49-kDa precursor protein which is expressed in intracellular stages of the parasite. The surface localization of gp40 and gp15 and their involvement in the host-parasite interaction suggest that either or both of these glycoproteins may serve as effective targets for specific preventive or therapeutic measures for cryptosporidiosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.