ArticleInfection and immunity2000
High intracellular level of guanosine tetraphosphate in Mycobacterium smegmatis changes the morphology of the bacterium.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 84 citations in OpenAlex.
- Age-Dependent Pleomorphism inMicroorganisms · 2025Article
- Tale of Twin Bifunctional Second Messenger (p)ppGpp Synthetases and Their Function in Mycobacteria.ACS omega · 2023Review
- Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- InJournal of bacteriology · 2022Article
- Clinically encountered growth phenotypes of tuberculosis-causing bacilli and theirFrontiers in cellular and infection microbiology · 2022Review
- Identification of a RelA/SpoT Homolog and Its Possible Role in the Accumulation of Astaxanthin inFrontiers in plant science · 2022Article
- Insights into the molecular determinants involved inVirulence · 2021Review
- Stringent Response in Mycobacteria: From Biology to Therapeutic Potential.Pathogens (Basel, Switzerland) · 2021Review
- RelZ-Mediated Stress Response inJournal of bacteriology · 2020Article
- Insights into the Physiology and Metabolism of a Mycobacterial Cell in an Energy-Compromised State.Journal of bacteriology · 2019Article
- The stringent response and Mycobacterium tuberculosis pathogenesis.Pathogens and disease · 2018Review
- Species-Specific Interactions of Arr with RplK Mediate Stringent Response in Bacteria.Journal of bacteriology · 2018Article
- The roles of RelA/(p)ppGpp in glucose-starvation induced adaptive response in the zoonotic Streptococcus suis.Scientific reports · 2016Article
- Regulation of Growth, Cell Shape, Cell Division, and Gene Expression by Second Messengers (p)ppGpp and Cyclic Di-GMP in Mycobacterium smegmatis.Journal of bacteriology · 2016Article
- Rel Is Required for Morphogenesis of Resting Cells in Mycobacterium smegmatis.Frontiers in microbiology · 2016Article
- Mild Nutrient Starvation Triggers the Development of a Small-Cell Survival Morphotype in Mycobacteria.Frontiers in microbiology · 2016Article
- The Borrelia burgdorferi RelA/SpoT Homolog and Stringent Response Regulate Survival in the Tick Vector and Global Gene Expression during Starvation.PLoS pathogens · 2015Article
- The bacterial alarmone (p)ppGpp activates the type III secretion system in Erwinia amylovora.Journal of bacteriology · 2015Article
- The application of tetracyclineregulated gene expression systems in the validation of novel drug targets in Mycobacterium tuberculosis.Frontiers in microbiology · 2015Review
- Essential roles for Mycobacterium tuberculosis Rel beyond the production of (p)ppGpp.Journal of bacteriology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Almost one-third of the world population today harbors the tubercle bacillus asymptomatically. It is postulated that the morphology and staining pattern of the long-term persistors are different from those of actively growing culture. Interestingly, it has been found that the morphology and staining pattern of the starved in vitro population of mycobacteria is similar to the persistors obtained from the lung lesions. In order to delineate the biochemical characteristics of starved mycobacteria, Mycobacteria smegmatis was grown in 0.2% glucose as a sole carbon source along with an enriched culture in 2% glucose. Accumulation of the stringent factor guanosine tetraphosphate (ppGpp) with a concomitant change in morphology was observed for M. smegmatis under carbon-deprived conditions. In addition, M. smegmatis assumed a coccoid morphology when ppGpp was ectopically produced by overexpressing Escherichia coli relA, even in an enriched medium. The Mycobacterium tuberculosis relA and spoT homologue, when induced in M. smegmatis, also resulted in the overproduction of ppGpp with a change in the bacterium's growth characteristics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.