ArticleInfection and immunity2000
Tuberculosis DNA vaccine encoding Ag85A is immunogenic and protective when administered by intramuscular needle injection but not by epidermal gene gun bombardment.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 87 citations in OpenAlex.
- BCG-derived acellular membrane vesicles elicit antimycobacterial immunity and innate immune memory.Frontiers in immunology · 2025Article
- Mycobacteriosis and Infections with Non-tuberculous Mycobacteria in Aquatic Organisms: A Review.Microorganisms · 2020Review
- Dendritic cell vaccine with Ag85A enhances anti-colorectal carcinoma immunity.Experimental and therapeutic medicine · 2018Article
- Down selecting adjuvanted vaccine formulations: a comparative method for harmonized evaluation.BMC immunology · 2018Article
- Immunogenicity and protective efficacy of a novel recombinant BCG strain overexpressing antigens Ag85A and Ag85B.Clinical & developmental immunology · 2012Article
- Immunogenicity and protective efficacy against murine tuberculosis of a prime-boost regimen with BCG and a DNA vaccine expressing ESAT-6 and Ag85A fusion protein.Clinical & developmental immunology · 2011Article
- Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Mycobacterium tuberculosis conserved hypothetical protein rRv2626c modulates macrophage effector functions.Immunology · 2010Article
- Ag85B DNA vaccine suppresses airway inflammation in a murine model of asthma.Respiratory research · 2009Article
- DNA vaccine constructs against enterovirus 71 elicit immune response in mice.Genetic vaccines and therapy · 2007Article
- DNA vaccine using Mycobacterium bovis Ag85B antigen induces partial protection against experimental infection in BALB/c mice.Clinical and vaccine immunology : CVI · 2006Article
- In vitro cellular immune responses to recombinant antigens of Mycobacterium avium subsp. paratuberculosis.Infection and immunity · 2005Article
- A second-generation anti TB vaccine is long overdue.Annals of clinical microbiology and antimicrobials · 2004Article
- Induction of protective cellular immunity against Mycobacterium tuberculosis by recombinant attenuated self-destructing Listeria monocytogenes strains harboring eukaryotic expression plasmids for antigen 85 complex and MPB/MPT51.Infection and immunity · 2004Article
- Immunogenicity of recombinant BCG vaccine strains overexpressing components of the antigen 85 complex of Mycobacterium tuberculosis.Medical microbiology and immunology · 2004Article
- Article
- On the use of DNA vaccines for the prophylaxis of mycobacterial diseases.Infection and immunity · 2003Review
- Vaccination of guinea pigs with DNA encoding the mycobacterial antigen MPB83 influences pulmonary pathology but not hematogenous spread following aerogenic infection with Mycobacterium bovis.Infection and immunity · 2002Article
- New hope for an AIDS vaccine.Nature reviews. Immunology · 2002Review
- Protection against woodchuck hepatitis virus (WHV) infection by gene gun coimmunization with WHV core and interleukin-12.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunogenicity and protective efficacy of a DNA vaccine encoding Ag85A from Mycobacterium tuberculosis were compared in BALB/c and C57BL (B6 and B10) mice immunized by intramuscular (i.m.) needle injection or epidermal gene gun (gg) bombardment. In BALB/c mice, gg immunization could induce elevated antibody and cytotoxic T lymphocyte responses with plasmid doses 50-fold lower than those required for i.m. immunization. Interleukin-2 (IL-2) and gamma interferon (IFN-gamma) secretion, however, was much lower in gg-immunized than in i.m.-immunized BALB/c mice. On the other hand, C57BL mice reacted only very weakly to gg immunization, whereas elevated Ag85A-specific antibody, IL-2, and IFN-gamma responses (significantly higher than in BALB/c mice) were detected following vaccination by the i.m. route. Antibody isotypes were indicative of Th2 activation following gg injection of BALB/c and of Th1 activation following i.m. injection of C57BL mice. Finally, C57BL but not BALB/c mice were protected by i.m. Ag85A DNA immunization against intravenous M. tuberculosis challenge, as measured by reduced numbers of CFU in spleen and lungs, compared to animals vaccinated with control DNA. Gene gun immunization was not effective in either BALB/c or C57BL mice. These results indicate that i.m. DNA vaccination is the method of choice for the induction of protective Th1 type immune responses with the Ag85A tuberculosis DNA vaccine.
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