ArticleInfection and immunity2000
Identification of three essential regulatory gene loci governing expression of Staphylococcus epidermidis polysaccharide intercellular adhesin and biofilm formation.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 129 citations in OpenAlex.
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- Staphylococcus epidermidis alters macrophage polarization and phagocytic uptake by extracellular DNA release in vitro.NPJ biofilms and microbiomes · 2024Article
- Biofilm-mediated infections by multidrug-resistant microbes: a comprehensive exploration and forward perspectives.Archives of microbiology · 2024Review
- Molecular Identification of Bacteria Isolated from MarketedLife (Basel, Switzerland) · 2023Article
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- The Transcription Factor SpoVG Is of Major Importance for Biofilm Formation ofInternational journal of molecular sciences · 2022Article
- Development of an artificial synovial fluid useful for studyingFrontiers in cellular and infection microbiology · 2022Article
- The impact of the superficial cuff position on the exit site and tunnel infections in CAPD patients.Journal of nephrology · 2021Article
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- Evaluation of methods to detect in vitro biofilm formation by staphylococcal clinical isolates.BMC research notes · 2018Article
- An 18 kDa scaffold protein is critical for Staphylococcus epidermidis biofilm formation.PLoS pathogens · 2015Article
- Staphylococcus epidermidis agr quorum-sensing system: signal identification, cross talk, and importance in colonization.Journal of bacteriology · 2014Article
- Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- An extracellular Staphylococcus epidermidis polysaccharide: relation to Polysaccharide Intercellular Adhesin and its implication in phagocytosis.BMC microbiology · 2012Article
- Genetic regulation of the intercellular adhesion locus in staphylococci.Frontiers in cellular and infection microbiology · 2012Review
- The eukaryotic-type serine/threonine protein kinase Stk is required for biofilm formation and virulence in Staphylococcus epidermidis.PloS one · 2011Article
- Understanding the significance of Staphylococcus epidermidis bacteremia in babies and children.Current opinion in infectious diseases · 2010Review
- Production of icaADBC-encoded polysaccharide intercellular adhesin and therapeutic failure in pediatric patients with Staphylococcal device-related infections.BMC infectious diseases · 2010Article
- The role of polysaccharide intercellular adhesin (PIA) in Staphylococcus epidermidis adhesion to host tissues and subsequent antibiotic tolerance.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of adherent multilayered biofilms embedded into a glycocalyx represents an essential factor in the pathogenesis of Staphylococcus epidermidis biomaterial-related infections. Using biofilm-producing S. epidermidis 1457 and transposon Tn917 carried on plasmid pTV1ts, we isolated nine isogenic biofilm-negative transposon mutants. Transduction by S. epidermidis phage 71 was used to prove the genetic linkage of transposon insertions and altered phenotypes. Mapping of the different transposon insertions by Southern hybridization and pulsed-field gel electrophoresis indicated that these were inserted in four unlinked genetic loci. According to their phenotypes, including quantitative differences in biofilm production in different growth media, in the amount of the polysaccharide intercellular adhesin (PIA) produced, in the hemagglutination titers, and in the altered colony morphology, the mutants could be separated into four phenotypic classes corresponding with the genetic classes. Synthesis of PIA was not detectable with class I and II mutants, whereas the amount of PIA produced reflected the residual degree of biofilm production of class III and IV mutants in different growth media. Chromosomal DNA flanking the transposon insertions of five class I mutants was cloned and sequenced, and the insertions were mapped to different locations of icaADBC, representing the synthetic genes for PIA. Expression of icaADBC from a xylose-dependent promoter in the different isogenic mutant classes reconstituted biofilm production in all mutants. In a Northern blot analysis no icaADBC-specific transcripts were observed in RNA isolated from mutants of classes II, III, and IV. Apparently, in addition to icaADBC, three other gene loci have a direct or indirect regulatory influence on expression of the synthetic genes for PIA on the level of transcription.
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