ArticleThe EMBO journal2000
Separate domains in E1 and E2 proteins serve architectural and productive roles for cooperative DNA binding.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Experimental Support for Human Papillomavirus Genome Amplification Early after Infectious Delivery.Journal of virology · 2023Article
- Human Papilloma Virus-Infected Cells.Sub-cellular biochemistry · 2023Article
- Using competition assays to quantitatively model cooperative binding by transcription factors and other ligands.Biochimica et biophysica acta. General subjects · 2017Article
- Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16.BMC cancer · 2017Article
- A conserved regulatory module at the C terminus of the papillomavirus E1 helicase domain controls E1 helicase assembly.Journal of virology · 2015Article
- The papillomavirus E2 proteins.Virology · 2013Review
- The E1 proteins.Virology · 2013Review
- Article
- Surface mutagenesis of the bovine papillomavirus E1 DNA binding domain reveals residues required for multiple functions related to DNA replication.Journal of virology · 2006Article
- Cellular topoisomerase I modulates origin binding by bovine papillomavirus type 1 E1.Journal of virology · 2006Article
- Papillomavirus E1 protein binds to and stimulates human topoisomerase I.Journal of virology · 2006Article
- Essential elements of a licensed, mammalian plasmid origin of DNA synthesis.Molecular and cellular biology · 2006Article
- Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen induces a strong bend on binding to terminal repeat DNA.Journal of virology · 2005Article
- The X-ray structure of the papillomavirus helicase in complex with its molecular matchmaker E2.Genes & development · 2004Article
- Direct interaction between nucleosome assembly protein 1 and the papillomavirus E2 proteins involved in activation of transcription.Molecular and cellular biology · 2004Article
- NFI and Oct-1 bend the Ad5 origin in the same direction leading to optimal DNA replication.Nucleic acids research · 2004Article
- Characterization of the minimal DNA binding domain of the human papillomavirus e1 helicase: fluorescence anisotropy studies and characterization of a dimerization-defective mutant protein.Journal of virology · 2003Article
- E1 initiator DNA binding specificity is unmasked by selective inhibition of non-specific DNA binding.The EMBO journal · 2003Article
- Sequential and ordered assembly of E1 initiator complexes on the papillomavirus origin of DNA replication generates progressive structural changes related to melting.Molecular and cellular biology · 2002Article
- Cooperative activation of human papillomavirus type 8 gene expression by the E2 protein and the cellular coactivator p300.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The E1 and E2 proteins from bovine papillomavirus bind cooperatively to binding sites in the viral origin of DNA replication. The DNA-binding domains (DBDs) of the two proteins interact with each other, and the E2 transactivation domain interacts with the helicase domain of E1. Mutations that disrupt the interaction between the two DBDs also disrupt the interaction between the E2 activation domain and the E1 helicase domain, demonstrating interdependence of the two interactions. Cooperative binding of the two DBDs generates a sharp bend in the DNA that is required for interaction between the E2 activation domain and E1. This indicates that interaction between the two DBDs plays an architectural role, 'triggering' a productive interaction between the E2 transactivation domain and E1 through introduction of a sharp bend in the DNA. This two-step mechanism may be a required feature for cooperative DNA binding to proximal binding sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.