ArticleThe EMBO journal2000
Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 131 citations in OpenAlex.
- The TPR2 corepressor forms condensates with repressors to fine-tune growth and development in rice.The EMBO journal · 2026Article
- Context-dependent interplay of Swi-Snf and Tup1-Cyc8 influences chromatin and transcription at antagonistically regulated genes.Nature communications · 2026Article
- Article
- Structural basis for C-degron selectivity across KLHDCX family E3 ubiquitin ligases.Nature communications · 2024Article
- Mechanism and evolutionary origins of alanine-tail C-degron recognition by E3 ligases Pirh2 and CRL2-KLHDC10.Cell reports · 2023Article
- Article
- Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1.Current genetics · 2023Article
- In silico modeling human VPS13 proteins associated with donor and target membranes suggests lipid transfer mechanisms.Proteins · 2023Article
- Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae.PLoS genetics · 2022Article
- Sublinear scaling of the cellular proteome with ploidy.Nature communications · 2022Article
- Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressor.Genetics · 2021Article
- Review
- Article
- Structural Basis of H2B Ubiquitination-Dependent H3K4 Methylation by COMPASS.Molecular cell · 2019Article
- Crystal structure of the WD40 domain dimer of LRRK2.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Recognition of the Diglycine C-End Degron by CRL2Molecular cell · 2018Article
- RNA-DNA sequence differences in Saccharomyces cerevisiae.Genome research · 2016Article
- The Unicellular Ancestry of Groucho-Mediated Repression and the Origins of Metazoan Transcription Factors.Genome biology and evolution · 2016Article
- Dynamic Protein Interaction Networks and New Structural Paradigms in Signaling.Chemical reviews · 2016Review
- Acquisition of the ability to assimilate mannitol by Saccharomyces cerevisiae through dysfunction of the general corepressor Tup1-Cyc8.Applied and environmental microbiology · 2015Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Tup1-Ssn6 corepressor complex regulates the expression of several sets of genes, including genes that specify mating type in the yeast Saccharomyces cerevisiae. Repression of mating-type genes occurs when Tup1-Ssn6 is brought to the DNA by the Matalpha2 DNA-binding protein and assembled upstream of a- and haploid-specific genes. We have determined the 2.3 A X-ray crystal structure of the C-terminal domain of Tup1 (accesion No. 1ERJ), a 43 kDa fragment that contains seven copies of the WD40 sequence motif and binds to the Matalpha2 protein. Moreover, this portion of the protein can partially substitute for full-length Tup1 in bringing about transcriptional repression. The structure reveals a seven-bladed beta propeller with an N-terminal subdomain that is anchored to the side of the propeller and extends the beta sheet of one of the blades. Point mutations in Tup1 that specifically affect the Tup1-Matalpha2 interaction cluster on one surface of the propeller. We identified regions of Tup1 that are conserved among the fungal Tup1 homologs and may be important in protein-protein interactions with additional components of the Tup1-mediated repression pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.